Mechanoenzymatic peptide and amide bond formation

José G. Hernández, Karen J. Ardila-Fierro, Deborah Crawford, Stuart James, Carsten Bolm

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


Mechanochemical chemoenzymatic peptide and amide bond formation catalysed by papain was studied by ball milling. Despite the high-energy mixing experienced inside the ball mill, the biocatalyst proved stable and highly efficient to catalyse the formation of α,α- and α,β-dipeptides. This strategy was further extended to the enzymatic acylation of amines by milling, and to the mechanosynthesis of a derivative of the valuable dipeptide L-alanyl-L-glutamine.
Original languageEnglish
Pages (from-to)2620-2625
JournalGreen Chemistry
Issue number11
Publication statusPublished - 02 May 2017


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