Abstract
3-Monochloropropane-1,2-diol (3-MCPD), glycidol, and their esters are some major sources of risk factors during food processing. Here we showed the biomarker analysis of 2,3-dihydroxypropyl mercapturic acid (DHPMA) isomers which derived from the metabolism of 3-MCPD, glycidol, and their esters in urine of rats and humans. Iso-DHPMA, a novel urinary metabolite, was discovered and detected in urine of rats, which were orally administered with glycidol but not 3-MCPD. Using the quadrupole-orbitrap high-resolution mass spectrometry, we confirmed that iso-DHPMA appeared a specific biomarker which derived from glycidol. The limit of quantification (signal-to-noise ratio, 10:1) of the analytes in urine of rats and humans were 1.33 ng/mL and 1.56 ng/mL, respectively. Acceptable within-laboratory reproducibility (RSD<9.0%) and spiking recovery (94.7%–100.1%) substantially supported the use of current method for robust biomarker analysis, which was successfully applied to the toxicokinetic study of DHPMA in rats and short-term internal exposure to 3-MCPD and glycidol in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 329-336 |
| Number of pages | 8 |
| Journal | Talanta |
| Volume | 204 |
| Early online date | 12 Jun 2019 |
| DOIs | |
| Publication status | Published - 01 Nov 2019 |
| Externally published | Yes |
Bibliographical note
Funding Information:We thank Waters Corporation for their technical support of mass spectrometry instrumentation. This work was financially supported by the National Key Research and Development Program of China (Grant No. 2017YFC1600500 ), National Natural Science Foundation of China (Grant No. 21677043 ) and China National Program for Support of Top-notch Young Professionals.
Publisher Copyright:
© 2019 Elsevier B.V.
Keywords
- 2,3-Dihydroxypropyl mercapturic acid
- 3-Monochloropropane-1,2-diol
- Glycidol
- Internal exposure
- Isomers
- Toxicokinetics
ASJC Scopus subject areas
- Analytical Chemistry