Metamaterials Nanosensor for Label-free Analysis of Conformation and Molecular Interaction of Biomolecules

Cuong Cao, Jun Zhang, Qihua Xiong

Research output: Contribution to conferencePoster

Abstract

Analysis of molecular interaction and conformational dynamics of biomolecules is of paramount importance in understanding of their vital functions in complex biological systems, disease detection, and new drug development. Plasmonic biosensors based upon surface plasmon resonance and localized surface plasmon resonance have become the predominant workhorse for detecting accumulated biomass caused by molecular binding events. However, unlike surface-enhanced Raman spectroscopy (SERS), the plasmonic biosensors indeed are not suitable tools to interrogate vibrational signatures of conformational transitions required for biomolecules to interact. Here, we show that plasmonic metamaterials can offer two transducing channels for parallel acquisition of optical transmission and sensitive SERS spectra at the biointerface, simultaneously probing the conformational states and binding affinity of biomolecules, e.g. G-quadruplexes, in different environments (Fig. 1). We further demonstrate the use of the metamaterials for fingerprinting and detection of arginine-glycine-glycine domain of nucleolin, a cancer biomarker which specifically binds to a G-quadruplex, with the picomolar sensitivity. The dual-mode nanosensor will significantly contribute to unraveling the complexes of the conformational dynamics of biomolecules as well as to improving specificity of biodetection assays.
Original languageEnglish
Publication statusPublished - 2012
EventSmall Science Symposium-Frontiers in Nanomedicine - Singapore , Singapore
Duration: 10 Dec 201212 Dec 2012

Conference

ConferenceSmall Science Symposium-Frontiers in Nanomedicine
CountrySingapore
CitySingapore
Period10/12/201212/12/2012

Fingerprint Dive into the research topics of 'Metamaterials Nanosensor for Label-free Analysis of Conformation and Molecular Interaction of Biomolecules'. Together they form a unique fingerprint.

  • Cite this

    Cao, C., Zhang, J., & Xiong, Q. (2012). Metamaterials Nanosensor for Label-free Analysis of Conformation and Molecular Interaction of Biomolecules. Poster session presented at Small Science Symposium-Frontiers in Nanomedicine, Singapore , Singapore. http://www3.ntu.edu.sg/symposium/3s2012/