Methodology for the at-home collection of urine samples for prostate cancer detection

Martyn Webb; Kate Manley; Mireia Olivan; Ingrid Guldvik; Malgorzata Palczynska; Rachel Hurst; Shea P Connell; Ian G Mills; Daniel S Brewer; Robert Mills; Colin S Cooper; Jeremy Clark

doi:10.2144/btn-2019-0092

Methodology for the at-home collection of urine samples for prostate cancer detection

Martyn Webb, Kate Manley, Mireia Olivan, Ingrid Guldvik, Malgorzata Palczynska, Rachel Hurst, Shea P Connell, Ian G Mills, Daniel S Brewer, Robert Mills, Colin S Cooper, Jeremy Clark

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Abstract

Urine from patients with prostate cancer (PCa) contains gene transcripts that have been used for PCa diagnosis and prognosis. Historically, patient urine samples have been collected after a digital rectal examination of the prostate, which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to those for post digital rectal examination urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home collection protocol has opened up the potential to perform large-scale PCa studies without the inconvenience, cost, discomfort and expense of patients having to visit the clinic.

Original language	English
Journal	BioTechniques
Early online date	29 Nov 2019
DOIs	https://doi.org/10.2144/btn-2019-0092
Publication status	Early online date - 29 Nov 2019

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10.2144/btn-2019-0092Licence: CC BY-NC-ND

Methodology for the at-home collection of urine samples for prostate cancer detection
Copyright 2019 the authors. This is an open access article published under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.
Final published version, 1.48 MBLicence: CC BY-NC-ND

Cite this

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title = "Methodology for the at-home collection of urine samples for prostate cancer detection",

abstract = "Urine from patients with prostate cancer (PCa) contains gene transcripts that have been used for PCa diagnosis and prognosis. Historically, patient urine samples have been collected after a digital rectal examination of the prostate, which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to those for post digital rectal examination urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home collection protocol has opened up the potential to perform large-scale PCa studies without the inconvenience, cost, discomfort and expense of patients having to visit the clinic.",

author = "Martyn Webb and Kate Manley and Mireia Olivan and Ingrid Guldvik and Malgorzata Palczynska and Rachel Hurst and Connell, {Shea P} and Mills, {Ian G} and Brewer, {Daniel S} and Robert Mills and Cooper, {Colin S} and Jeremy Clark",

year = "2019",

month = nov,

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doi = "10.2144/btn-2019-0092",

language = "English",

journal = "BioTechniques",

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TY - JOUR

T1 - Methodology for the at-home collection of urine samples for prostate cancer detection

AU - Webb, Martyn

AU - Manley, Kate

AU - Olivan, Mireia

AU - Guldvik, Ingrid

AU - Palczynska, Malgorzata

AU - Hurst, Rachel

AU - Connell, Shea P

AU - Mills, Ian G

AU - Brewer, Daniel S

AU - Mills, Robert

AU - Cooper, Colin S

AU - Clark, Jeremy

PY - 2019/11/29

Y1 - 2019/11/29

N2 - Urine from patients with prostate cancer (PCa) contains gene transcripts that have been used for PCa diagnosis and prognosis. Historically, patient urine samples have been collected after a digital rectal examination of the prostate, which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to those for post digital rectal examination urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home collection protocol has opened up the potential to perform large-scale PCa studies without the inconvenience, cost, discomfort and expense of patients having to visit the clinic.

AB - Urine from patients with prostate cancer (PCa) contains gene transcripts that have been used for PCa diagnosis and prognosis. Historically, patient urine samples have been collected after a digital rectal examination of the prostate, which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to those for post digital rectal examination urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home collection protocol has opened up the potential to perform large-scale PCa studies without the inconvenience, cost, discomfort and expense of patients having to visit the clinic.

U2 - 10.2144/btn-2019-0092

DO - 10.2144/btn-2019-0092

M3 - Article

C2 - 31779479

SN - 0736-6205

JO - BioTechniques

JF - BioTechniques

ER -

Methodology for the at-home collection of urine samples for prostate cancer detection

Abstract

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