Microbial decolorization and bioremediation of anaerobically digested molasses spent wash effluent by aerobic bacterial cultures

Vinod Kumar, L. Wati, P. Nigam*, Ibrahim M. Banat, G. McMullan, Dave Singh, R. Marchant

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Anaerobically digested molasses spent wash (DMSW) is a dark-brown-coloured recalcitrant effluent which has a high chemical oxygen demand (COD) and high pollution potential. Bacterial enrichment cultures were set up to obtain isolates capable of DMSW decolorization and bioremediation. Two aerobic bacterial cultures LA1 and D-2 were obtained using soil samples from an Indian distillery plant. Both grew well on 12.5% diluted digested spent wash supplemented with glucose as a readily available carbon source. The optimum conditions for growth and DMSW decolorization were 35°C, pH 7.0, 3% glucose (w/v) and 0.5% peptone or yeast extract (w/v) in a basal mineral salt medium. The maximum decolorization (36.5% and 32.5%) and COD reduction (41.0% and 39.0%) were achieved for cultures LA1 and D-2, respectively, after 8 days incubation under the stated conditions. The advantages of using such a culture in digested spent wash bioremediation are discussed.

Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalMicrobios
Volume89
Issue number359
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • Bioremediation
  • Decolorization
  • Digested spent wash
  • Distillery effluent
  • Molasses
  • Waste water

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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