Microfluidic-Mediated self-assembly of Phospholipids for the delivery of Biologic Molecules

Edward Weaver, Edward O’Connor, David Cole, Andy Hooker, Shahid Uddin, Dimitrios Lamprou*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The encapsulation of biologic molecules using a microfluidic platform is a procedure that has been understudied but shows great promise from initial reported studies. The study focusses upon the encapsulation of bovine serum albumin (BSA) under various parameters and using multiple phospholipids to identify optimal conditions for the manufacturing of protein loaded lipid nanoparticles. Additionally, encapsulation of the enzyme trypsin (TRP) has been investigated to show the eligibility of the system to other biological medications. All liposomes were subject to rigorous physicochemical characterisation, including differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR), to document the successful synthesis of the liposomes. Drug-loaded liposome stability was investigated over a 28-day period at 5°C and 37°C, which showed encouraging results for 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at all concentrations of BSA used. The sample containing 1 mg/ml BSA grew by only 10% over the study, which considering liposomes should be affected highly by biologic adsorption, shows great promise for the formulations. Encapsulation and in vitro release studies showed improved loading capacity for BSA compared to conventional methods, whilst maintaining a concise controlled release of the active pharmaceutical ingredient (API).
Original languageEnglish
JournalInternational Journal of Pharmaceutics
Publication statusAccepted - 30 Nov 2021

Keywords

  • Microfluidics
  • Liposomes
  • nanomedicines
  • Biologics
  • Peptides
  • drug delivery

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