Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide

Cristina L Marolda; Piya Lahiry; Enrique Vinés; Soledad Saldías; Miguel A Valvano

doi:10.1385/1-59745-167-3:237

Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide

Cristina L Marolda, Piya Lahiry, Enrique Vinés, Soledad Saldías, Miguel A Valvano

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

90 Citations (Scopus)

Abstract

Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.

Original language	English
Pages (from-to)	237-52
Number of pages	16
Journal	Methods in Molecular Biology
Volume	347
DOIs	https://doi.org/10.1385/1-59745-167-3:237
Publication status	Published - 2006

ASJC Scopus subject areas

Genetics
Molecular Biology

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abstract = "Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.",

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AU - Lahiry, Piya

AU - Vinés, Enrique

AU - Saldías, Soledad

AU - Valvano, Miguel A

PY - 2006

Y1 - 2006

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AB - Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.

U2 - 10.1385/1-59745-167-3:237

DO - 10.1385/1-59745-167-3:237

M3 - Article

C2 - 17072014

SN - 1064-3745

VL - 347

SP - 237

EP - 252

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide

Abstract

ASJC Scopus subject areas

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