Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide

Cristina L Marolda, Piya Lahiry, Enrique Vinés, Soledad Saldías, Miguel A Valvano

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.
Original languageEnglish
Pages (from-to)237-52
Number of pages16
JournalMethods in Molecular Biology
Volume347
DOIs
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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