Microneedle mediated intradermal delivery of adjuvanted recombinant HIV-1 CN54gp140 effectively primes mucosal boost inoculations

Aditya Pattani, Paul F. McKay, Martin J. Garland, Rhonda Curran, Katarzyna Migalska, Corona M. Cassidy, R. Karl Malcolm, Robin J. Shattock, Helen O. McCarthy, Ryan F. Donnelly

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)
222 Downloads (Pure)

Abstract

Dissolving polymeric microneedle arrays formulated to contain recombinant CN54 HIVgp140 and the TLR4 agonist adjuvant MPLA were assessed for their ability to elicit antigen-specific immunity. Using this novel microneedle system we successfully primed antigen-specific responses that were further boosted by an intranasal mucosal inoculation to elicit significant antigen-specific immunity. This prime-boost modality generated similar serum and mucosal gp140-specific IgG levels to the adjuvanted and systemic subcutaneous inoculations. While the microneedle primed groups demonstrated a balanced Th1/Th2 profile, strong Th2 polarization was observed in the subcutaneous inoculation group, likely due to the high level of IL-5 secretion from cells in this group. Significantly, the animals that received a microneedle prime and intranasal boost regimen elicited a high level IgA response in both the serum and mucosa, which was greatly enhanced over the subcutaneous group. The splenocytes from this inoculation group secreted moderate levels of IL-5 and IL-10 as well as high amounts of IL-2, cytokines known to act in synergy to induce IgA. This work opens up the possibility for microneedle-based HIV vaccination strategies that, once fully developed, will greatly reduce risk for vaccinators and patients, with those in the developing world set to benefit most.
Original languageEnglish
Pages (from-to)529-537
Number of pages9
JournalJournal of Controlled Release
Volume162
Issue number3
DOIs
Publication statusPublished - 28 Sep 2012

Keywords

  • HIV
  • Vaccination
  • Microneedle
  • Intranasal
  • Subcutaneous

ASJC Scopus subject areas

  • Pharmaceutical Science

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