Microneedle-mediated transdermal bacteriophage delivery

Elizabeth Ryan, Martin J. Garland, Thakur Raghu Raj Singh, Eoin Bambury, John O'Dea, Katarzyna Migalska, Sean P. Gorman, Helen O. McCarthy, Brendan F. Gilmore, Ryan F. Donnelly

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Interest in bacteriophages as therapeutic agents has recently been reawakened. Parenteral delivery is the most routinely-employed method of administration. However, injection of phages has numerous disadvantages, such as the requirement of a health professional for administration and the possibility of cross-contamination. Transdermal delivery offers one potential means of overcoming many of these problems. The present study utilized a novel poly (carbonate) (PC) hollow microneedle (MN) device for the transdermal delivery of Escherichia coli-specific 14 bacteriophages both in vitro and in vivo. MN successfully achieved bacteriophage delivery in vitro across dermatomed and full thickness skin. A concentration of 2.67 x 10(6) PFU/ml (plaque forming units per ml) was detected in the receiver compartment when delivered across dermatomed skin and 4.0 x 10(3) PFU/ml was detected in the receiver compartment when delivered across full thickness skin. An in vivo study resulted in 4.13 x 10(3) PFU/ml being detected in blood 30 min following initial MN-mediated phage administration. Clearance occurred rapidly, with phages being completely cleared from the systemic circulation within 24 h, which was expected in the absence of infection. We have shown here that MN-mediated delivery allows successful systemic phage absorption. Accordingly, bacteriophage-based therapeutics may now have an alternative route for systemic delivery. Once fully-investigated, this could lead to more widespread investigation of these interesting therapeutic viruses. (c) 2012 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalEuropean journal of pharmaceutical sciences
Volume47
Issue number2
DOIs
Publication statusPublished - 29 Sep 2012

Keywords

  • Bacteriophage therapy
  • Transdermal delivery
  • Hollow microneedle
  • Bacterial infection

ASJC Scopus subject areas

  • Pharmaceutical Science

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