Microsatellite loci for basking shark (Cetorhinus maximus) monitoring and conservation

Lilian Lieber; Deborah A.  Dawson; Gavin J. Horsburgh; Leslie R. Noble; Catherine S. Jones

doi:10.1007/s12686-015-0493-8

Microsatellite loci for basking shark (Cetorhinus maximus) monitoring and conservation

Lilian Lieber^*, Deborah A. Dawson, Gavin J. Horsburgh, Leslie R. Noble, Catherine S. Jones^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

The filter-feeding basking shark (Cetorhinus maximus) is the largest fish found in the Northeast Atlantic, however there is little baseline ecological data despite its globally threatened status. In order to investigate the basking sharks’ population connectivity, patterns of relatedness and effective population numbers over time, we developed a microsatellite marker set suitable for genetic
monitoring. Forty-seven markers were screened, of which 24 were assessed in 20 unrelated individuals from the Isle of Man using DNA extracted from skin mucus swabs. We selected 19 polymorphic loci with a mean observed heterozygosity of 0.73 to create a marker set with sufficient power to unambiguously resolve between individuals (PIsibs=1.7 x10^-7).

Original language	English
Pages (from-to)	917–944
Journal	Conservation Genetics Resources
Volume	7
Issue number	4
DOIs	https://doi.org/10.1007/s12686-015-0493-8
Publication status	Published - 17 Nov 2015

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Microsatellite loci for basking shark (Cetorhinus maximus) monitoring and conservation
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title = "Microsatellite loci for basking shark (Cetorhinus maximus) monitoring and conservation",

abstract = "The filter-feeding basking shark (Cetorhinus maximus) is the largest fish found in the Northeast Atlantic, however there is little baseline ecological data despite its globally threatened status. In order to investigate the basking sharks{\textquoteright} population connectivity, patterns of relatedness and effective population numbers over time, we developed a microsatellite marker set suitable for geneticmonitoring. Forty-seven markers were screened, of which 24 were assessed in 20 unrelated individuals from the Isle of Man using DNA extracted from skin mucus swabs. We selected 19 polymorphic loci with a mean observed heterozygosity of 0.73 to create a marker set with sufficient power to unambiguously resolve between individuals (PIsibs=1.7 x10-7). ",

author = "Lilian Lieber and Dawson, {Deborah A.} and Horsburgh, {Gavin J.} and Noble, {Leslie R.} and Jones, {Catherine S.}",

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AU - Lieber, Lilian

AU - Dawson, Deborah A.

AU - Horsburgh, Gavin J.

AU - Noble, Leslie R.

AU - Jones, Catherine S.

PY - 2015/11/17

Y1 - 2015/11/17

N2 - The filter-feeding basking shark (Cetorhinus maximus) is the largest fish found in the Northeast Atlantic, however there is little baseline ecological data despite its globally threatened status. In order to investigate the basking sharks’ population connectivity, patterns of relatedness and effective population numbers over time, we developed a microsatellite marker set suitable for geneticmonitoring. Forty-seven markers were screened, of which 24 were assessed in 20 unrelated individuals from the Isle of Man using DNA extracted from skin mucus swabs. We selected 19 polymorphic loci with a mean observed heterozygosity of 0.73 to create a marker set with sufficient power to unambiguously resolve between individuals (PIsibs=1.7 x10-7).

AB - The filter-feeding basking shark (Cetorhinus maximus) is the largest fish found in the Northeast Atlantic, however there is little baseline ecological data despite its globally threatened status. In order to investigate the basking sharks’ population connectivity, patterns of relatedness and effective population numbers over time, we developed a microsatellite marker set suitable for geneticmonitoring. Forty-seven markers were screened, of which 24 were assessed in 20 unrelated individuals from the Isle of Man using DNA extracted from skin mucus swabs. We selected 19 polymorphic loci with a mean observed heterozygosity of 0.73 to create a marker set with sufficient power to unambiguously resolve between individuals (PIsibs=1.7 x10-7).

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DO - 10.1007/s12686-015-0493-8

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JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

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ER -

Microsatellite loci for basking shark (Cetorhinus maximus) monitoring and conservation

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