Hypoxia modulates reparative angiogenesis, which is a tightly regulated pathophysiological process. MicroRNAs (miRNAs) are important regulators of gene expression in hypoxia and angiogenesis. However, we do not yet have a clear understanding of how hypoxia-induced miRNAs fine-tune vasoreparative processes. Here, we identify miR-130a as a mediator of the hypoxic response in human primary endothelial colony-forming cells (ECFCs), a well-characterized subtype of endothelial progenitors. Under hypoxic conditions of 1% O , miR-130a gain-of-function enhances ECFC pro-angiogenic capacity and potentiates their vasoreparative properties . Mechanistically, miR-130a orchestrates upregulation of VEGFR2, activation of STAT3, and accumulation of HIF1α via translational inhibition of . These findings unveil a new role for miR-130a in hypoxia, whereby it activates the VEGFR2/STAT3/HIF1α axis to enhance the vasoregenerative capacity of ECFCs.
|Number of pages||14|
|Journal||Molecular Therapy: Nucleic Acids|
|Early online date||20 Jan 2021|
|Publication status||Published - 05 Mar 2021|
Bibliographical noteCrown Copyright © 2021.
- endothelial cell
- endothelial progenitor
- vascular repair
FingerprintDive into the research topics of 'miR-130a activates the VEGFR2/STAT3/HIF1α axis to potentiate the vasoregenerative capacity of endothelial colony-forming cells in hypoxia'. Together they form a unique fingerprint.
Vasogenic bloodborne progenitors and diabetesAuthor: McLoughlin, K., Jul 2020
Supervisor: Medina, R. (Supervisor), Lois, N. (Supervisor) & Stitt, A. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy