Misonidazole binding in murine liver tissue: a marker for cellular hypoxia in vivo

A. P. Maxwell, M. P. MacManus, T. A. Gardiner

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The hepatic microcirculation is believed to cause variable cellular oxygenation within the organ. In this study a marker of cellular hypoxia was used to demonstrate liver oxygen tension gradients in vivo. Covalent binding of misonidazole adducts to cellular macromolecules is enhanced by hypoxia. Autoradiographs of liver from mice treated with radiolabeled misonidazole demonstrated enhanced binding of adducts within hepatocytes surrounding hepatic veins. Livers from both hypoxic and normal mice had characteristic autoradiographic grain patterns reflecting regional oxygen tension variation in vivo. Differential binding of misonidazole adducts formed in hypoxic cells could have an application in studies of liver physiology and biochemistry.
Original languageUndefined/Unknown
Pages (from-to)1300-1303
Number of pages4
JournalGastroenterology
Volume97
Issue number5
Publication statusPublished - 01 Nov 1989

Bibliographical note

LR: 20061115; JID: 0374630; 0 (Carbon Radioisotopes); 10028-17-8 (Tritium); 13551-87-6 (Misonidazole); 7782-44-7 (Oxygen); ppublish

Keywords

  • Animals
  • Autoradiography
  • Carbon Radioisotopes/diagnostic use
  • Cell Hypoxia
  • Liver/metabolism
  • Mice
  • Mice, Inbred Strains
  • Misonidazole/pharmacokinetics
  • Oxygen/metabolism
  • Tritium/diagnostic use

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