Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China

Yin Ping DONG; Wei WANG; Tao JIANG; Jin XU; Chun Hui HAN; Shao Fei YAN; Séamus Fanning; Ying LI; Xiao Chen MA; Di ZHANG; Yao ZHAO; Biao ZENG; Feng Qin LI

doi:10.3967/bes2017.061

Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China

Yin Ping DONG, Wei WANG, Tao JIANG, Jin XU, Chun Hui HAN, Shao Fei YAN, Séamus Fanning, Ying LI, Xiao Chen MA, Di ZHANG, Yao ZHAO, Biao ZENG, Feng Qin LI^*

^*Corresponding author for this work

Research output: Contribution to journal › Letter › peer-review

7 Citations (Scopus)

Abstract

Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula (PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins (BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis (PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores.

Original language	English
Pages (from-to)	460-464
Number of pages	5
Journal	Biomedical and Environmental Sciences
Volume	30
Issue number	6
DOIs	https://doi.org/10.3967/bes2017.061
Publication status	Published - 01 Jun 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 The Editorial Board of Biomedical and Environmental Sciences

Keywords

China
Clostridium botulinum
Infant botulism
Powdered infant formula

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3967/bes2017.061Licence: Unspecified

Cite this

@article{ae8e2aa6e0b9443db815d0f86d1d3389,

title = "Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China",

abstract = "Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula (PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins (BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis (PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores.",

keywords = "China, Clostridium botulinum, Infant botulism, Powdered infant formula",

author = "DONG, {Yin Ping} and Wei WANG and Tao JIANG and Jin XU and HAN, {Chun Hui} and YAN, {Shao Fei} and S{\'e}amus Fanning and Ying LI and MA, {Xiao Chen} and Di ZHANG and Yao ZHAO and Biao ZENG and LI, {Feng Qin}",

note = "Publisher Copyright: {\textcopyright} 2017 The Editorial Board of Biomedical and Environmental Sciences",

year = "2017",

month = jun,

day = "1",

doi = "10.3967/bes2017.061",

language = "English",

volume = "30",

pages = "460--464",

journal = "Biomedical and Environmental Sciences",

issn = "0895-3988",

publisher = "Elsevier B.V.",

number = "6",

}

TY - JOUR

T1 - Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China

AU - DONG, Yin Ping

AU - WANG, Wei

AU - JIANG, Tao

AU - XU, Jin

AU - HAN, Chun Hui

AU - YAN, Shao Fei

AU - Fanning, Séamus

AU - LI, Ying

AU - MA, Xiao Chen

AU - ZHANG, Di

AU - ZHAO, Yao

AU - ZENG, Biao

AU - LI, Feng Qin

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula (PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins (BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis (PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores.

AB - Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula (PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins (BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis (PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores.

KW - China

KW - Clostridium botulinum

KW - Infant botulism

KW - Powdered infant formula

U2 - 10.3967/bes2017.061

DO - 10.3967/bes2017.061

M3 - Letter

C2 - 28705271

AN - SCOPUS:85026451309

SN - 0895-3988

VL - 30

SP - 460

EP - 464

JO - Biomedical and Environmental Sciences

JF - Biomedical and Environmental Sciences

IS - 6

ER -

Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Fingerprint

Cite this