Molecular-assisted immunohistochemical optimization

M.F.M. Omar, N. Huang, K. Ou, K. Yu, T.C. Putti, H. Jikuya, T. Ichikawa, O. Nishimura, P. Tan, Manuel Salto-Tellez

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Immunohistochemistry (IHC) is an essential tool in diagnostic surgical pathology, allowing analysis of protein subcellular localization The use of IHC by different laboratories has lead to inconsistencies in published literature for several antibodies, due to either interpretative (inter-observer venation) or technical reasons These disparities have major implications in both clinical and research settings In this study, we report our experience conducting an IHC optimization of antibodies against five proteins previously identified by proteomic analysis to be breast cancer biomarkers, namely 6PGL (PGLS), CAZ2 (CAPZA2), PA2G4 (EBP1) PSD2 and TKT Large variations in the immunolocalizations and intensities were observed when manipulating the antigen retrieval method and primary antibody incubation concentration However, the use of an independent molecular analysis method provided a clear indication in choosing the appropriate biologically and functionally relevant
Original languageEnglish
Pages (from-to)519-528
Number of pages10
JournalActa Histochemica
Volume112
Issue number6
DOIs
Publication statusPublished - Nov 2010

ASJC Scopus subject areas

  • Cell Biology
  • Histology

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