Molecular characterization of blaESBL-producing Escherichia coli cultured from pig farms in Ireland

Juan Wang, James F. Gibbons, Kathleen McGrath, Li Bai, Fengqin Li, Finola C. Leonard, Roger Stephan, Séamus Fanning*

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives
To characterize ESBL-encoding Escherichia coli cultured from pigs and their plasmids carrying these genes following conjugation into recipient strains.

Methods
Six ESBL-producing E. coli were recovered from faecal samples taken from pigs along with a further isolate from the environment of a farrowing house on three pig farms in Ireland. These isolates were characterized by phylogenetic grouping, MLST and ESBL genotype analyses. Conjugation experiments were carried out in broth mating assays. S1-nuclease PFGE was used to determine the plasmid profiles. Whole-genome sequences of the seven E. coli were determined and subsequently analysed.

Results
Phylogenetic groups and the corresponding MLST STs identified among the seven tested E. coli isolates included A/ST10, A/ST34, C/ST23 and C/ST1629. All seven isolates carried one or more high-molecular-weight plasmids and demonstrated the ability to transfer their cefotaxime resistance phenotype at high frequencies. Five transmissible plasmid replicon types were detected, including IncK/B (n = 3), IncI1 (n = 2), IncFIA (n = 1), IncFIB (n = 1) and IncN (n = 1). ESBL-encoding genes, including blaCTX-M-14, blaCTX-M-15 and blaTEM-20, were identified.

Conclusions
As the first report from pig sources in Ireland, characterization of these ESBL-encoding isolates and their transmissible plasmids extends our understanding on these resistance markers from porcine E. coli.
Original languageEnglish
Article numberdkw278
Pages (from-to)3062-3065
JournalJournal of Antimicrobial Chemotherapy
Volume71
Issue number11
DOIs
Publication statusPublished - 25 Jul 2016

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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    Wang, J., Gibbons, J. F., McGrath, K., Bai, L., Li, F., Leonard, F. C., Stephan, R., & Fanning, S. (2016). Molecular characterization of blaESBL-producing Escherichia coli cultured from pig farms in Ireland. Journal of Antimicrobial Chemotherapy, 71(11), 3062-3065. [dkw278]. https://doi.org/10.1093/jac/dkw278