Molecular characterization of complete and incomplete immunoglobulin heavy chain gene rearrangements in hairy cell leukemia.

P. Martín-Jiménez, R. García-Sanz, D. González, A. Balanzategui, J.J. Pérez, M.D. Caballero, M.E. Sarasquete, J. Galende, A. Orfao, M.C. López-Berges, J.F. San Miguel, M. González

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8 Citations (Scopus)

Abstract

PURPOSE: We analyzed patients with hairy cell leukemia (HCL) to achieve a better understanding of the differentiation stage reached by HCL cells and to define the key role of the diversification of cell surface makers, especially CD25 expression. PATIENTS AND METHODS: We analyzed 38 previously untreated patients with HCL to characterize their complete (VDJ(H)) and incomplete (DJ(H)) immunoglobulin (Ig) heavy chain (IgH) rearrangements, including somatic hypermutation pattern and gene segment use. RESULTS: A correlation between immunophenotypic profile and molecular data was seen. All 38 cases showed monoclonal amplifications: VDJ(H) in 97%, DJ(H) in 42%, and both in 39%. Segments from the D(H)3 family were used more in complete compared with incomplete rearrangements (45% vs. 12%; P <.005). Furthermore, comparison between molecular and immunophenotypic characteristics disclosed differences in the expression of CD25 antigen; CD25(-) cases, a phenotype associated with HCL variant, showed complete homology to the germline in 3 of 5 cases (60%), whereas this characteristic was never observed in CD25(+) cases (P <.005). Moreover, V(H)4-34, V(H)1-08, and J(H)3 segments appeared in 2, 1, and 2 CD25(-) cases, respectively, whereas they were absent in all CD25(+) cases. CONCLUSION: These results support that HCL is a heterogeneous entity including subgroups with different molecular characteristics, which reinforces the need for additional studies with a larger number of patients to clarify the real role of gene rearrangements in HCL.
Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalCLINICAL LYMPHOMA & MYELOMA
Volume7
Issue number9
Publication statusPublished - Nov 2007

Keywords

  • Gene Rearrangement
  • Genome, Human
  • Humans
  • Immunoglobulin Heavy Chains
  • Immunophenotyping
  • Leukemia, Hairy Cell
  • Mutation
  • VDJ Exons

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