Molecular characterization of Nosema bombi (Microsporidia : Nosematidae) and a note on its sites of infection in Bombus terrestris (Hymenoptera : Apoidea)

I. Fries, A. De Ruijter, Robert Paxton, A.J. Da Silva, S.B. Slemenda, N.J. Pieniazek

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Investigations of queen, worker and male bumble bees (Bombus terrestris) showed that all individuals became infected with Nosema bombi. Infections were found in Malpighian tubules, thorax muscles, fat body tissue and nerve tissue, including the brain. Ultrastructural studies revealed thin walled emptied spores in host cell cytoplasm interpreted as autoinfective spores, besides normal spores (environmental spores) intended for parasite transmission between hosts. The nucleotide sequence of the gene coding for the small subunit rRNA (SSU-rRNA) from Microsporidia isolated from B. terrestris, B. lucorum, and B. hortorum were identical, providing evidence that N. bombi infects multiple hosts. The sequence presented here (GenBank Accession no AY008373) is different from an earlier submission to GenBank (Accession no U26158) of a partial sequence of the same gene based on material collected from B. terrestris. It still remains to be investigated if there is species diversity among Microsporidia found in bumble bees.
Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalJOURNAL OF APICULTURAL RESEARCH
Volume40
Issue number3-4
Publication statusPublished - 2001

Fingerprint

Nosema
Bombus terrestris
Microsporidia
Apoidea
spores
Hymenoptera
infection
nucleotide sequences
Malpighian tubules
fat body
thorax
queen insects
nerve tissue
cytoplasm
ribosomal RNA
brain
parasites
species diversity
muscles
genes

Cite this

Fries, I. ; De Ruijter, A. ; Paxton, Robert ; Da Silva, A.J. ; Slemenda, S.B. ; Pieniazek, N.J. / Molecular characterization of Nosema bombi (Microsporidia : Nosematidae) and a note on its sites of infection in Bombus terrestris (Hymenoptera : Apoidea). In: JOURNAL OF APICULTURAL RESEARCH. 2001 ; Vol. 40, No. 3-4. pp. 91-96.
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Molecular characterization of Nosema bombi (Microsporidia : Nosematidae) and a note on its sites of infection in Bombus terrestris (Hymenoptera : Apoidea). / Fries, I.; De Ruijter, A.; Paxton, Robert; Da Silva, A.J.; Slemenda, S.B.; Pieniazek, N.J.

In: JOURNAL OF APICULTURAL RESEARCH, Vol. 40, No. 3-4, 2001, p. 91-96.

Research output: Contribution to journalArticle

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