Molecular detection of CF lung pathogens current status and future potential

Sally H Pattison, Geraint B Rogers, Martin Crockard, J Stuart Elborn, Michael M Tunney

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Molecular diagnostic tests, based on the detection and identification of nucleic acids in human biological samples, are increasingly employed in the diagnosis of infectious diseases and may be of future benefit to CF microbiology services. Our growing understanding of the complex polymicrobial nature of CF airway infection has highlighted current and likely future shortcomings in standard diagnostic practices. Failure to detect fastidious or slow growing microbes and misidentification of newly emerging pathogens could potentially be addressed using culture-independent molecular technologies with high target specificity. This review considers existing molecular diagnostic tests in the context of the key requirements for an envisaged CF microbiology focussed assay. The issues of assay speed, throughput, detection of multiple pathogens, data interpretation and antimicrobial susceptibility testing are discussed.
Original languageEnglish
Pages (from-to)194-205
JournalJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
Issue number3
Publication statusPublished - May 2013

Bibliographical note

Copyright © 2013 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.


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