Molecular diversity of the HLA-C gene identified in a Caucasian population

F. Williams, A. Meenagh, Christopher Patterson, Derek Middleton

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A DNA typing procedure, based on a two stage polymerase chain reaction-sequence-specific oligonucleotide probe (PCR-SSOP) typing strategy, has been developed and applied to DNA from 1000 healthy individuals from the Northern Ireland region. The two-stage procedure involves human leukocyte antigen (HLA-C) identification through the use of a medium resolution PCR-SSOP system, followed by four secondary group specific PCR-SSOP systems, to enable allele resolution. The PCR-SSOP systems were designed for the identification of HLA-Cw alleles with possible discrimination within exons 2 and 3 of the HLA-C gene, i.e., HLA-Cw*01-Cw*16. PCR-SSP tests were designed for the resolution of HLA-Cw*17 and -Cw*18 alleles. The systems can also be used independently of each other if selective allele resolution is required. HLA-Cw allele frequencies occuring within the Northern Ireland population have been compiled, along with estimations of HLA-B/Cw haplotype frequencies. (C) American Society for Histocompatibility and Immunogenetics, 2002. Published by Elsevier Science Inc.
Original languageEnglish
Pages (from-to)602-613
Number of pages12
JournalHuman Immunology
Volume63
Issue number7
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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    Williams, F., Meenagh, A., Patterson, C., & Middleton, D. (2002). Molecular diversity of the HLA-C gene identified in a Caucasian population. Human Immunology, 63(7), 602-613.