Selective androgen receptor modulators (SARMs) are non-steroidal compounds widely reported as drugs of abuse in human and animal sports, with potential for misuse as growth promoters in animal-based food production. In this study, a first analytical methodology to simultaneous screen for a panel of emerging SARMs in bovine muscle was developed, validated (CCβ values from 0.5–5 ng g−1), and applied to detect 15 structurally diverse compounds from nine SARM families. Muscle samples (200 mg) were homogenised in extraction solvent (MeCN:H2O, 4:1, v/v) before clean-up (end-capped C18 dSPE), defatting (n-hexane pre-saturated with MeCN partitioning) and concentration prior to UHPLC-MS/MS analysis. In the absence of incurred bovine muscle, method applicability was demonstrated by the analysis of rodent muscle tissue. The developed screening assay serves as a rapid, simple and cost-effective tool for surveillance monitoring of SARM abuse in livestock production systems as a pre-emptive measure ensuring food safety.
- Food safety
- Muscle tissue
- Residue screening
- Selective androgen receptor modulators
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science
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Development and validation of ultra-high performance liquid chromatography-tandem mass spectrometry methods for screening of selective androgen receptor modulators in urine and bloodAuthor: Ventura, E., Jul 2020
Student thesis: Doctoral Thesis › Doctor of PhilosophyFile