Monitoring of selective androgen receptor modulators in bovine muscle tissue by ultra-high performance liquid chromatography-tandem mass spectrometry

Anna Gadaj; Emiliano Ventura; Alexis Ripoche; Mark H. Mooney

doi:10.1016/j.fochx.2019.100056

Monitoring of selective androgen receptor modulators in bovine muscle tissue by ultra-high performance liquid chromatography-tandem mass spectrometry

Anna Gadaj^*, Emiliano Ventura, Alexis Ripoche, Mark H. Mooney

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

222 Downloads (Pure)

Abstract

Selective androgen receptor modulators (SARMs) are non-steroidal compounds widely reported as drugs of abuse in human and animal sports, with potential for misuse as growth promoters in animal-based food production. In this study, a first analytical methodology to simultaneous screen for a panel of emerging SARMs in bovine muscle was developed, validated (CCβ values from 0.5–5 ng g⁻¹), and applied to detect 15 structurally diverse compounds from nine SARM families. Muscle samples (200 mg) were homogenised in extraction solvent (MeCN:H₂O, 4:1, v/v) before clean-up (end-capped C₁₈ dSPE), defatting (n-hexane pre-saturated with MeCN partitioning) and concentration prior to UHPLC-MS/MS analysis. In the absence of incurred bovine muscle, method applicability was demonstrated by the analysis of rodent muscle tissue. The developed screening assay serves as a rapid, simple and cost-effective tool for surveillance monitoring of SARM abuse in livestock production systems as a pre-emptive measure ensuring food safety.

Original language	English
Article number	100056
Number of pages	8
Journal	Food Chemistry: X
Volume	4
Early online date	24 Sep 2019
DOIs	https://doi.org/10.1016/j.fochx.2019.100056
Publication status	Published - 30 Dec 2019

Keywords

Food safety
Muscle tissue
Residue screening
SARMs
Selective androgen receptor modulators
UHPLC-MS/MS

ASJC Scopus subject areas

Analytical Chemistry
Food Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.fochx.2019.100056Licence: CC BY-NC-ND

Monitoring of selective androgen receptor modulators in bovine muscle tissue by ultra-high performance liquid chromatography-tandem mass spectrometry
© 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
Final published version, 1.24 MBLicence: CC BY-NC-ND

Cite this

@article{05a931ee38944325818a604c08e8b393,

title = "Monitoring of selective androgen receptor modulators in bovine muscle tissue by ultra-high performance liquid chromatography-tandem mass spectrometry",

abstract = "Selective androgen receptor modulators (SARMs) are non-steroidal compounds widely reported as drugs of abuse in human and animal sports, with potential for misuse as growth promoters in animal-based food production. In this study, a first analytical methodology to simultaneous screen for a panel of emerging SARMs in bovine muscle was developed, validated (CCβ values from 0.5–5 ng g−1), and applied to detect 15 structurally diverse compounds from nine SARM families. Muscle samples (200 mg) were homogenised in extraction solvent (MeCN:H2O, 4:1, v/v) before clean-up (end-capped C18 dSPE), defatting (n-hexane pre-saturated with MeCN partitioning) and concentration prior to UHPLC-MS/MS analysis. In the absence of incurred bovine muscle, method applicability was demonstrated by the analysis of rodent muscle tissue. The developed screening assay serves as a rapid, simple and cost-effective tool for surveillance monitoring of SARM abuse in livestock production systems as a pre-emptive measure ensuring food safety.",

keywords = "Food safety, Muscle tissue, Residue screening, SARMs, Selective androgen receptor modulators, UHPLC-MS/MS",

author = "Anna Gadaj and Emiliano Ventura and Alexis Ripoche and Mooney, {Mark H.}",

year = "2019",

month = dec,

day = "30",

doi = "10.1016/j.fochx.2019.100056",

language = "English",

volume = "4",

journal = "Food Chemistry: X",

issn = "2590-1575",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Monitoring of selective androgen receptor modulators in bovine muscle tissue by ultra-high performance liquid chromatography-tandem mass spectrometry

AU - Gadaj, Anna

AU - Ventura, Emiliano

AU - Ripoche, Alexis

AU - Mooney, Mark H.

PY - 2019/12/30

Y1 - 2019/12/30

N2 - Selective androgen receptor modulators (SARMs) are non-steroidal compounds widely reported as drugs of abuse in human and animal sports, with potential for misuse as growth promoters in animal-based food production. In this study, a first analytical methodology to simultaneous screen for a panel of emerging SARMs in bovine muscle was developed, validated (CCβ values from 0.5–5 ng g−1), and applied to detect 15 structurally diverse compounds from nine SARM families. Muscle samples (200 mg) were homogenised in extraction solvent (MeCN:H2O, 4:1, v/v) before clean-up (end-capped C18 dSPE), defatting (n-hexane pre-saturated with MeCN partitioning) and concentration prior to UHPLC-MS/MS analysis. In the absence of incurred bovine muscle, method applicability was demonstrated by the analysis of rodent muscle tissue. The developed screening assay serves as a rapid, simple and cost-effective tool for surveillance monitoring of SARM abuse in livestock production systems as a pre-emptive measure ensuring food safety.

AB - Selective androgen receptor modulators (SARMs) are non-steroidal compounds widely reported as drugs of abuse in human and animal sports, with potential for misuse as growth promoters in animal-based food production. In this study, a first analytical methodology to simultaneous screen for a panel of emerging SARMs in bovine muscle was developed, validated (CCβ values from 0.5–5 ng g−1), and applied to detect 15 structurally diverse compounds from nine SARM families. Muscle samples (200 mg) were homogenised in extraction solvent (MeCN:H2O, 4:1, v/v) before clean-up (end-capped C18 dSPE), defatting (n-hexane pre-saturated with MeCN partitioning) and concentration prior to UHPLC-MS/MS analysis. In the absence of incurred bovine muscle, method applicability was demonstrated by the analysis of rodent muscle tissue. The developed screening assay serves as a rapid, simple and cost-effective tool for surveillance monitoring of SARM abuse in livestock production systems as a pre-emptive measure ensuring food safety.

KW - Food safety

KW - Muscle tissue

KW - Residue screening

KW - SARMs

KW - Selective androgen receptor modulators

KW - UHPLC-MS/MS

UR - http://www.scopus.com/inward/record.url?scp=85072996220&partnerID=8YFLogxK

U2 - 10.1016/j.fochx.2019.100056

DO - 10.1016/j.fochx.2019.100056

M3 - Article

AN - SCOPUS:85072996220

VL - 4

JO - Food Chemistry: X

JF - Food Chemistry: X

SN - 2590-1575

M1 - 100056

ER -

Monitoring of selective androgen receptor modulators in bovine muscle tissue by ultra-high performance liquid chromatography-tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Development and validation of ultra-high performance liquid chromatography-tandem mass spectrometry methods for screening of selective androgen receptor modulators in urine and blood

Cite this

Monitoring of selective androgen receptor modulators in bovine muscle tissue by ultra-high performance liquid chromatography-tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Student theses

Development and validation of ultra-high performance liquid chromatography-tandem mass spectrometry methods for screening of selective androgen receptor modulators in urine and blood

Cite this