MTERF1 Binds mtDNA to Prevent Transcriptional Interference at the Light-Strand Promoter but Is Dispensable for rRNA Gene Transcription Regulation

Mügen Terzioglu, Benedetta Ruzzenente, Julia Harmel, Arnaud Mourier, Elisabeth Jemt, Marcela Dávila López, Christian Kukat, James B. Stewart, Rolf Wibom, Caroline Meharg, Bianca Habermann, Maria Falkenberg, Claes M Gustafsson, Chan Bae Park, Nils-Göran Larsson

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)

Abstract

Mitochondrial transcription termination factor 1, MTERF1, has been reported to couple rRNA gene transcription initiation with termination and is therefore thought to be a key regulator of mammalian mitochondrial ribosome biogenesis. The prevailing model is based on a series of observations published over the last two decades, but no in vivo evidence exists to show that MTERF1 regulates transcription of the heavy-strand region of mtDNA containing the rRNA genes. Here, we demonstrate that knockout of Mterf1 in mice has no effect on mitochondrial rRNA levels or mitochondrial translation. Instead, loss of Mterf1 influences transcription initiation at the light-strand promoter, resulting in a decrease of de novo transcription manifested as reduced 7S RNA levels. Based on these observations, we suggest that MTERF1 does not regulate heavy-strand transcription, but rather acts to block transcription on the opposite strand of mtDNA to prevent transcription interference at the light-strand promoter.
Original languageEnglish
Pages (from-to)618-26
Number of pages9
JournalCell metabolism
Volume17
Issue number4
DOIs
Publication statusPublished - 02 Apr 2013

Bibliographical note

Copyright © 2013 Elsevier Inc. All rights reserved.

Fingerprint

Dive into the research topics of 'MTERF1 Binds mtDNA to Prevent Transcriptional Interference at the Light-Strand Promoter but Is Dispensable for rRNA Gene Transcription Regulation'. Together they form a unique fingerprint.

Cite this