Multi sulfonamide screening in porcine muscle using a surface plasmon resonance biosensor

T McGrath*, A Baxter, J Ferguson, S Haughey, P Bjurling

*Corresponding author for this work

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A binding protein displaying broad-spectrum cross-reactivity within the sulfonamide group was used in conjunction with a sulfonamide specific sensor chip and a surface plasmon resonance biosensor to develop a rapid broad spectrum screening assay for sulfonamides in porcine muscle. Results for 40 samples were available in just over 5 h after the completion of a simple sample preparation protocol. Twenty sulfonamide compounds were detected. Acetylated metabolites were not recognised by the binding protein. Limit of detection (mean-three times standard deviation value when n = 20) was calculated to be 16.9 ng g(-1) in tissue samples. Intra-assay precision (n = 10) was calculated at 4.3 %CV for a sample spiked at 50 ng g(-1) with sulfamethazine, 3.6 %CV for a sample spiked at 100 ng g(-1) with sulfamethazine, 7.2 %CV for a sample spiked at 50 ng g(-1) with sulfadiazine and 3.1 %CV for a sample spiked at 100 ng g-1 with sulfadiazine. Inter-assay precision (n = 3) was calculated at 9.7 %CV for a sample spiked at 50 ng g-1 with sulfamethazine, 3.8 %CV for a sample spiked at 100 ng g(-1) with sulfamethazine, 3.5 %CV for a sample spiked at 50 ng g(-1) with sulfadiazine and 2.8 %CV for a sample spiked at 100 ng g(-1) with sulfadiazine. (C) 2004 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalAnalytica Chimica Acta
Volume529
Issue number1-2
DOIs
Publication statusPublished - 24 Jan 2005
Event5th EURORESIDUE Conference - Noordwijkerhout, Netherlands
Duration: 10 May 200412 May 2004

Keywords

  • MILK
  • SULFADIAZINE
  • SPR biosensor
  • screening test
  • ASSAY
  • sulfamethazine sulfadiazine
  • pork muscle
  • ELISA
  • generic sulfonamides assay
  • SULFAMETHAZINE RESIDUES

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