Abstract
A binding protein displaying broad-spectrum cross-reactivity within the sulfonamide group was used in conjunction with a sulfonamide specific sensor chip and a surface plasmon resonance biosensor to develop a rapid broad spectrum screening assay for sulfonamides in porcine muscle. Results for 40 samples were available in just over 5 h after the completion of a simple sample preparation protocol. Twenty sulfonamide compounds were detected. Acetylated metabolites were not recognised by the binding protein. Limit of detection (mean-three times standard deviation value when n = 20) was calculated to be 16.9 ng g(-1) in tissue samples. Intra-assay precision (n = 10) was calculated at 4.3 %CV for a sample spiked at 50 ng g(-1) with sulfamethazine, 3.6 %CV for a sample spiked at 100 ng g(-1) with sulfamethazine, 7.2 %CV for a sample spiked at 50 ng g(-1) with sulfadiazine and 3.1 %CV for a sample spiked at 100 ng g-1 with sulfadiazine. Inter-assay precision (n = 3) was calculated at 9.7 %CV for a sample spiked at 50 ng g-1 with sulfamethazine, 3.8 %CV for a sample spiked at 100 ng g(-1) with sulfamethazine, 3.5 %CV for a sample spiked at 50 ng g(-1) with sulfadiazine and 2.8 %CV for a sample spiked at 100 ng g(-1) with sulfadiazine. (C) 2004 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 123-127 |
Number of pages | 5 |
Journal | Analytica Chimica Acta |
Volume | 529 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 24 Jan 2005 |
Event | 5th EURORESIDUE Conference - Noordwijkerhout, Netherlands Duration: 10 May 2004 → 12 May 2004 |
Keywords
- MILK
- SULFADIAZINE
- SPR biosensor
- screening test
- ASSAY
- sulfamethazine sulfadiazine
- pork muscle
- ELISA
- generic sulfonamides assay
- SULFAMETHAZINE RESIDUES