Multilaboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens

Linda Stewart, Lyanne McCallan, James McNair, Adrian McGoldrick, Rowan Morris, Jean-Louis Moyen, Lucia De Juan Ferre, Beatriz Romero, Elena Alonso, Sven Parsons, Paul Van Helden, Flabio Araujo, Irene Grant

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Abstract

A recently developed lateral flow immunochromatographic device (LFD) for detection of Mycobacterium bovis cells was comprehensively evaluated for applications in veterinary diagnostic laboratories in different parts of the world. The LFD specifically detected M. bovis in the broadest sense (var bovis, var caprae and BCG) and, of a wide range of Mycobacterium spp. tested, cross-reacted only with one strain of M. pinnepedii. Analytical sensitivity and specificity of the novel LFD were excellent when the test was used to confirm the presence of M. bovis in MGIT liquid cultures or the identity of suspect colonies isolated on Coletsos, Lowenstein-Jensen or Stonebrink media from a variety of veterinary specimens. Although some bovine lymph node homogenates tested positive for M. bovis by the LFD, the test lacks sufficient analytical sensitivity to be a rapid method to directly detect M. bovis in veterinary diagnostic samples without prior culture. There was significant correlation between results obtained with the commercially available BD MGIT™ TBc ID test and the novel M. bovis-specific LFD (r = 0.9791; P<0.0001) applied to 190 MGIT cultures of bovine specimens, with the added benefit that the novel LFD differentiated M. bovis from another member of the M. tuberculosis complex in one positive culture. This multi-laboratory evaluation of the novel LFD test has clearly demonstrated the assay’s unique specificity for M. bovis and its potential utility as a rapid and inexpensive test to confirm isolation of M. bovis from veterinary specimens following liquid culture, or to confirm suspect colonies after solid culture.
Original languageEnglish
Pages (from-to)3411-3425
Number of pages15
JournalJournal of Clinical Microbiology
Volume55
Issue number12
Early online date27 Nov 2017
DOIs
Publication statusEarly online date - 27 Nov 2017

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    Stewart, L., McCallan, L., McNair, J., McGoldrick, A., Morris, R., Moyen, J-L., De Juan Ferre, L., Romero, B., Alonso, E., Parsons, S., Van Helden, P., Araujo, F., & Grant, I. (2017). Multilaboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens. Journal of Clinical Microbiology, 55(12), 3411-3425. https://doi.org/10.1128/JCM.00728-17