Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors

N. Rubio; L M Hirvonen; E Z Chong; J T W Wang; M Bourgognon; H Kafa; Hatem A F M Hassan; W T Al-Jamal; David McCarthy; C Hogstrand; F  Festy; Khuloud T Al-Jamal

doi:10.1039/c5cc02675j

Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors

N. Rubio, L M Hirvonen, E Z Chong, J T W Wang, M Bourgognon, H Kafa, Hatem A F M Hassan, W T Al-Jamal, David McCarthy, C Hogstrand, F Festy, Khuloud T Al-Jamal

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Abstract

The intrinsic nonlinear photoluminescence (PL) property of chemically functionalized multi-walled nanotubes MWNTs (f-MWNTs) is reported in this study. f-MWNTs are imaged in fixed lung epithelial cancer cells (A549) and Kupffer cells in vitro, and in subcutaneously implanted solid tumors in vivo, for the first time, using multiphoton PL and fluorescence lifetime imaging (FLIM). Multiphoton imaging in the near-infrared excitation region (∼750-950 nm), employed in this study in a label-free manner, provides sensitivity and resolution optimal to track f-MWNTs within intra-cellular compartments and facilitates tumour imaging and sentinel lymph node tracking in vivo. Wider applications include employing this technique in live imaging of f-MWNTs in biological milieu to facilitate image-guided drug delivery.

Original language	English
Pages (from-to)	9366-9369
Journal	Chemical Communications
Volume	51
Issue number	45
DOIs	https://doi.org/10.1039/c5cc02675j
Publication status	Published - 07 Jun 2015

Keywords

Animals
Cell Line, Tumor
Colonic Neoplasms
Diagnostic Imaging
Drug Delivery Systems
Humans
Luminescence
Mice
Nanotubes, Carbon
Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c5cc02675jLicence: CC BY

Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors†
Copyright 2015 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 2.46 MBLicence: CC BY

Wafa Al-Jamal
- w.al-jamalqub.acuk
- School of Pharmacy - Professor
- Material and Advanced Technologies for Healthcare
Person: Academic

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Rubio, N., Hirvonen, L. M., Chong, E. Z., Wang, J. T. W., Bourgognon, M., Kafa, H., Hassan, H. A. F. M., Al-Jamal, W. T., McCarthy, D., Hogstrand, C., Festy, F., & Al-Jamal, K. T. (2015). Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors. Chemical Communications, 51(45), 9366-9369. https://doi.org/10.1039/c5cc02675j

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title = "Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors",

abstract = "The intrinsic nonlinear photoluminescence (PL) property of chemically functionalized multi-walled nanotubes MWNTs (f-MWNTs) is reported in this study. f-MWNTs are imaged in fixed lung epithelial cancer cells (A549) and Kupffer cells in vitro, and in subcutaneously implanted solid tumors in vivo, for the first time, using multiphoton PL and fluorescence lifetime imaging (FLIM). Multiphoton imaging in the near-infrared excitation region (∼750-950 nm), employed in this study in a label-free manner, provides sensitivity and resolution optimal to track f-MWNTs within intra-cellular compartments and facilitates tumour imaging and sentinel lymph node tracking in vivo. Wider applications include employing this technique in live imaging of f-MWNTs in biological milieu to facilitate image-guided drug delivery.",

keywords = "Animals, Cell Line, Tumor, Colonic Neoplasms, Diagnostic Imaging, Drug Delivery Systems, Humans, Luminescence, Mice, Nanotubes, Carbon, Journal Article",

author = "N. Rubio and Hirvonen, {L M} and Chong, {E Z} and Wang, {J T W} and M Bourgognon and H Kafa and Hassan, {Hatem A F M} and Al-Jamal, {W T} and David McCarthy and C Hogstrand and F Festy and Al-Jamal, {Khuloud T}",

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doi = "10.1039/c5cc02675j",

language = "English",

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AU - Rubio, N.

AU - Hirvonen, L M

AU - Chong, E Z

AU - Wang, J T W

AU - Bourgognon, M

AU - Kafa, H

AU - Hassan, Hatem A F M

AU - Al-Jamal, W T

AU - McCarthy, David

AU - Hogstrand, C

AU - Festy, F

AU - Al-Jamal, Khuloud T

PY - 2015/6/7

Y1 - 2015/6/7

N2 - The intrinsic nonlinear photoluminescence (PL) property of chemically functionalized multi-walled nanotubes MWNTs (f-MWNTs) is reported in this study. f-MWNTs are imaged in fixed lung epithelial cancer cells (A549) and Kupffer cells in vitro, and in subcutaneously implanted solid tumors in vivo, for the first time, using multiphoton PL and fluorescence lifetime imaging (FLIM). Multiphoton imaging in the near-infrared excitation region (∼750-950 nm), employed in this study in a label-free manner, provides sensitivity and resolution optimal to track f-MWNTs within intra-cellular compartments and facilitates tumour imaging and sentinel lymph node tracking in vivo. Wider applications include employing this technique in live imaging of f-MWNTs in biological milieu to facilitate image-guided drug delivery.

AB - The intrinsic nonlinear photoluminescence (PL) property of chemically functionalized multi-walled nanotubes MWNTs (f-MWNTs) is reported in this study. f-MWNTs are imaged in fixed lung epithelial cancer cells (A549) and Kupffer cells in vitro, and in subcutaneously implanted solid tumors in vivo, for the first time, using multiphoton PL and fluorescence lifetime imaging (FLIM). Multiphoton imaging in the near-infrared excitation region (∼750-950 nm), employed in this study in a label-free manner, provides sensitivity and resolution optimal to track f-MWNTs within intra-cellular compartments and facilitates tumour imaging and sentinel lymph node tracking in vivo. Wider applications include employing this technique in live imaging of f-MWNTs in biological milieu to facilitate image-guided drug delivery.

KW - Animals

KW - Cell Line, Tumor

KW - Colonic Neoplasms

KW - Diagnostic Imaging

KW - Drug Delivery Systems

KW - Humans

KW - Luminescence

KW - Mice

KW - Nanotubes, Carbon

KW - Journal Article

U2 - 10.1039/c5cc02675j

DO - 10.1039/c5cc02675j

M3 - Article

C2 - 25959229

SN - 1359-7345

VL - 51

SP - 9366

EP - 9369

JO - Chemical Communications

JF - Chemical Communications

IS - 45

ER -

Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors

Abstract

Keywords

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Wafa Al-Jamal

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