Multiple mycotoxin exposure determined by urinary biomarkers in rural subsistence farmers in the former Transkei, South Africa

Gordon S Shephard, Hester-Mari Burger, Lucia Gambacorta, Yun Yun Gong, Rudolf Krska, John P Rheeder, Michele Solfrizzo, Chou Srey, Michael Sulyok, Angelo Visconti, Benedikt Warth, Liana van der Westhuizen

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Abstract

Subsistence farmers are exposed to a range of mycotoxins. This study applied novel urinary multi-mycotoxin LC-MS/MS methods to determine multiple exposure biomarkers in the high oesophageal cancer region, Transkei, South Africa. Fifty-three female participants donated part of their maize-based evening meal and first void morning urine, which was analysed both with sample clean-up (single and multi-biomarker) and by a 'dilute-and-shoot' multi-biomarker method. Results were corrected for recovery with LOD for not detected. A single biomarker method detected fumonisin B1 (FB1) (87% incidence; mean±standard deviation 0.342±0.466 ng/mg creatinine) and deoxynivalenol (100%; mean 20.4±49.4 ng/mg creatinine) after hydrolysis with β-glucuronidase. The multi-biomarker 'dilute-and-shoot' method indicated deoxynivalenol-15-glucuronide was predominantly present. A multi-biomarker method with β-glucuronidase and immunoaffinity clean-up determined zearalenone (100%; 0.529±1.60 ng/mg creatinine), FB1 (96%; 1.52±2.17 ng/mg creatinine), α-zearalenol (92%; 0.614±1.91 ng/mg creatinine), deoxynivalenol (87%; 11.3±27.1 ng/mg creatinine), β-zearalenol (75%; 0.702±2.95 ng/mg creatinine) and ochratoxin A (98%; 0.041±0.086 ng/mg creatinine). These demonstrate the value of multi-biomarker methods in measuring exposures in populations exposed to multiple mycotoxins. This is the first finding of urinary deoxynivalenol, zearalenone, their conjugates, ochratoxin A and zearalenols in Transkei.

Original languageEnglish
Pages (from-to)217-25
Number of pages9
JournalFood and Chemical Toxicology
Volume62
Early online date25 Aug 2013
DOIs
Publication statusPublished - Dec 2013

Bibliographical note

Copyright © 2013 Elsevier Ltd. All rights reserved.

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    Shephard, G. S., Burger, H-M., Gambacorta, L., Gong, Y. Y., Krska, R., Rheeder, J. P., Solfrizzo, M., Srey, C., Sulyok, M., Visconti, A., Warth, B., & van der Westhuizen, L. (2013). Multiple mycotoxin exposure determined by urinary biomarkers in rural subsistence farmers in the former Transkei, South Africa. Food and Chemical Toxicology, 62, 217-25. https://doi.org/10.1016/j.fct.2013.08.040