TY - JOUR
T1 - Multiple origins of European populations of giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), an invasive liver parasite of ruminants
AU - Králová-Hromadová, I.
AU - Bazsalovicsová, E.
AU - Štefka, J.
AU - Špakulová, M.
AU - Vávrová, S.
AU - Szemes, T.
AU - Tkach, V.
AU - Trudgett, Alan
AU - Pybus, M.
PY - 2011
Y1 - 2011
N2 - The giant liver fluke, Fascioloides magna, liver parasite of free-living and domestic ruminants
of Europe and North America, was analysed in order to determine the origin of European populations
and to reveal the biogeography of this originally North American parasite on the European continent.
The previously selected variable fragments of the mitochondrial cytochrome c oxidase subunit I (cox1;
384 bp) and nicotinamid dehydrogenase subunit I (nad1; 405 bp) were applied as a tool. The
phylogenetic trees and haplotype networks were constructed and the level of genetic structuring was
evaluated using population genetic tools. In F. magna individuals originating from all European natural
foci (Italy, Czech Republic, Danube floodplain forests) and from four of five major North American
enzootic areas, 16 cox1 and 18 nad1 haplotypes were determined. The concatenated sequence set
produced 22 distinct haplotypes. The European fluke populations were less diverse than those from
North America in that they contained proportionately fewer haplotypes (8), while more substantial
level of genetic diversity and higher number of haplotypes (15) were recorded in North America. Only
one haplotype was shared between the European (Italy) and North American (USA/Oregon and
Canada/Alberta) flukes supporting a western North American origin of the Italian F. magna population.
Haplotypes found in Italy were distinct from those determined in the remaining European localities
what indicates that introduction of F. magna onto the European continent is a result of more than one
event. In Czech focus, a south-eastern US origin of giant liver fluke was revealed. Identical haplotypes,
common for parasites from Czech Republic and from expanding focus of Danube floodplain forests,
implies introduction of F. magna to the Danube region from an already established Czech focus.
AB - The giant liver fluke, Fascioloides magna, liver parasite of free-living and domestic ruminants
of Europe and North America, was analysed in order to determine the origin of European populations
and to reveal the biogeography of this originally North American parasite on the European continent.
The previously selected variable fragments of the mitochondrial cytochrome c oxidase subunit I (cox1;
384 bp) and nicotinamid dehydrogenase subunit I (nad1; 405 bp) were applied as a tool. The
phylogenetic trees and haplotype networks were constructed and the level of genetic structuring was
evaluated using population genetic tools. In F. magna individuals originating from all European natural
foci (Italy, Czech Republic, Danube floodplain forests) and from four of five major North American
enzootic areas, 16 cox1 and 18 nad1 haplotypes were determined. The concatenated sequence set
produced 22 distinct haplotypes. The European fluke populations were less diverse than those from
North America in that they contained proportionately fewer haplotypes (8), while more substantial
level of genetic diversity and higher number of haplotypes (15) were recorded in North America. Only
one haplotype was shared between the European (Italy) and North American (USA/Oregon and
Canada/Alberta) flukes supporting a western North American origin of the Italian F. magna population.
Haplotypes found in Italy were distinct from those determined in the remaining European localities
what indicates that introduction of F. magna onto the European continent is a result of more than one
event. In Czech focus, a south-eastern US origin of giant liver fluke was revealed. Identical haplotypes,
common for parasites from Czech Republic and from expanding focus of Danube floodplain forests,
implies introduction of F. magna to the Danube region from an already established Czech focus.
U2 - 10.1016/j.ijpara.2010.10.010
DO - 10.1016/j.ijpara.2010.10.010
M3 - Article
SN - 0020-7519
VL - 41
SP - 373
EP - 383
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 3-4
ER -