Multiplex RT-PCR for the detection of leukemia-associated translocations - Validation and application to routine molecular diagnostic practice

Manuel Salto-Tellez, S.G. Shelat, B. Benoit, H. Rennert, M. Carroll, D.G.B. Leonard, P. Nowell, A. Bagg

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

The aim of this study was to validate the application of a commercially available multiplex reverse transcription polymerase chain reaction (RT-PCR) assay [He-mavision-7 System] for the seven most common leukemia translocations for routine molecular diagnostic hematopathology practice. A total of 98 samples, comprising four groups, were evaluated: Group 1, 16 diagnostic samples molecularly positive by our existing laboratory-developed assays for PML-RARalpha/t (15; 17) or BCR-ABL/t (9;22); Group 2, 51 diagnostic samples negative by our laboratory-developed assays for PML-RARalpha/t (15;17) or BCR-ABL/t (9;22); Group 3, 21 prospectively analyzed diagnostic cases, without prior molecular studies; and Group 4, 10 minimal residual disease (MRD) samples. Analysis of the two previously studied cohorts (Groups 1 and 2) confirmed the diagnostic sensitivity and specificity of the multiplex assay with regard to these two translocations. Additionally, however, in the
Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalJournal of Molecular Diagnostics
Volume5
Issue number4
Publication statusPublished - Nov 2003

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint Dive into the research topics of 'Multiplex RT-PCR for the detection of leukemia-associated translocations - Validation and application to routine molecular diagnostic practice'. Together they form a unique fingerprint.

Cite this