Mutations in the H, F, or M proteins can facilitate resistance of measles virus to neutralizing human anti-MV sera

Hasan Kweder, Michelle Ainouze, Sara Louise Cosby, Claude P. Muller, Camille Lévy, Els Verhoeyen, Francois-Loic Cosset, Evelyne Manet, Robin Buckland

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although there is currently no evidence of emerging strains of measles virus (MV) that can resist neutralization by the anti-MV antibodies present in vaccinees, certain mutations in circulating wt MV strains appear to reduce the efficacy of these antibodies. Moreover, it has been hypothesized that resistance to neutralization by such antibodies could allow MV to persist. In this study, we use a novel in vitro system to determine the molecular basis of MV's resistance to neutralization. We find that both wild-type and laboratory strain MV variants that escape neutralization by anti-MV polyclonal sera possess multiple mutations in their H, F, and M proteins. Cytometric analysis of cells expressing viral escape mutants possessing minimal mutations and their plasmid-expressed H, F, and M proteins indicates that immune resistance is due to particular mutations that can occur in any of these three proteins that affect at distance, rather than directly, the native conformation of the MV-H globular head and hence its epitopes. A high percentage of the escape mutants contain mutations found in cases of Subacute Sclerosing Panencephalitis (SSPE) and our results could potentially shed light on the pathogenesis of this rare fatal disease.
Original languageEnglish
Article number205617
Number of pages18
JournalAdvances in Virology
Volume2014
DOIs
Publication statusPublished - 04 Feb 2014

Keywords

  • measles
  • mutations
  • neutralising antibody

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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