Mycobiota of silk-faced ancient Mogao Grottoes manuscripts belonging to the Stein collection in the British library

Celso Martins, Cristina Silva Pereira, Natalia V. Plechkova*, Kenneth R. Seddon, Jianlan Wang, Susan Whitfield, Wingyui Wong

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)
46 Downloads (Pure)

Abstract

Silking, a conservation technique which involved gluing silk gauze over the face of a manuscript was popular in the mid-20th Century, especially for treating early Chinese documents. The method is now little used, and the question as to whether silking interventions should be reversed is controversial, given the high economic cost of active intervention, and there are few scientific studies as to the long-term consequences of the technique. Silk-facing materials from documents of the Stein collection were analysed using scanning electron microscopy coupled with energy dispersive X-ray spectroscopy. The mycobiota diversity was unravelled through the combination of culture dependent methods and amplicon sequencing analyses. The SEM micrographs showed smooth regular nodules of ca. 3–5 μm diameter on both silk threads and glue paste. This morphology differs from the irregular and the crystalline morphologies of glue paste and inorganic crystallites, respectively, but it is consistent with that of small-sized conidia (asexual spores of fungi) or yeasts. Glue paste demonstrated three fungal strains: Aspergillus tubingensis, Penicillium crustosum and Chrysonilia sitophila which display cellulolytic activity, except for the last one. Amplicon sequencing revealed that silk threads and glue paste host distinct mycobiota. Here, we preliminary show that the silking method may be affecting the overall integrity of the silk-faced manuscripts, principally due to contamination with cellulolytic fungal strains. Unless the silk facing is removed, irreversible damage to the documents is highly probable.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalInternational Biodeterioration and Biodegradation
Volume134
Early online date08 Aug 2018
DOIs
Publication statusPublished - Oct 2018

Fingerprint

Silk
Manuscripts
Libraries
Adhesive pastes
Glues
Ointments
Adhesives
scanning electron microscopy
X-Ray Emission Spectrometry
X-ray spectroscopy
yeast
Gluing
spore
Facings
Scanning electron microscopy
Fungal Spores
Aspergillus
Penicillium
fungus
Spores

Keywords

  • Amplicon sequencing analyses
  • Cellulolytic activity
  • Conidia
  • Conservation science
  • Culture dependent methods
  • Energy dispersive X-ray spectroscopy (EDAX)
  • Manuscripts
  • Mycobiota
  • Scanning Electron Microscopy (SEM)
  • Silking
  • The Stein collection

Cite this

Martins, Celso ; Silva Pereira, Cristina ; Plechkova, Natalia V. ; Seddon, Kenneth R. ; Wang, Jianlan ; Whitfield, Susan ; Wong, Wingyui. / Mycobiota of silk-faced ancient Mogao Grottoes manuscripts belonging to the Stein collection in the British library. In: International Biodeterioration and Biodegradation. 2018 ; Vol. 134. pp. 1-6.
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abstract = "Silking, a conservation technique which involved gluing silk gauze over the face of a manuscript was popular in the mid-20th Century, especially for treating early Chinese documents. The method is now little used, and the question as to whether silking interventions should be reversed is controversial, given the high economic cost of active intervention, and there are few scientific studies as to the long-term consequences of the technique. Silk-facing materials from documents of the Stein collection were analysed using scanning electron microscopy coupled with energy dispersive X-ray spectroscopy. The mycobiota diversity was unravelled through the combination of culture dependent methods and amplicon sequencing analyses. The SEM micrographs showed smooth regular nodules of ca. 3–5 μm diameter on both silk threads and glue paste. This morphology differs from the irregular and the crystalline morphologies of glue paste and inorganic crystallites, respectively, but it is consistent with that of small-sized conidia (asexual spores of fungi) or yeasts. Glue paste demonstrated three fungal strains: Aspergillus tubingensis, Penicillium crustosum and Chrysonilia sitophila which display cellulolytic activity, except for the last one. Amplicon sequencing revealed that silk threads and glue paste host distinct mycobiota. Here, we preliminary show that the silking method may be affecting the overall integrity of the silk-faced manuscripts, principally due to contamination with cellulolytic fungal strains. Unless the silk facing is removed, irreversible damage to the documents is highly probable.",
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Mycobiota of silk-faced ancient Mogao Grottoes manuscripts belonging to the Stein collection in the British library. / Martins, Celso; Silva Pereira, Cristina; Plechkova, Natalia V.; Seddon, Kenneth R.; Wang, Jianlan; Whitfield, Susan; Wong, Wingyui.

In: International Biodeterioration and Biodegradation, Vol. 134, 10.2018, p. 1-6.

Research output: Contribution to journalArticle

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T1 - Mycobiota of silk-faced ancient Mogao Grottoes manuscripts belonging to the Stein collection in the British library

AU - Martins, Celso

AU - Silva Pereira, Cristina

AU - Plechkova, Natalia V.

AU - Seddon, Kenneth R.

AU - Wang, Jianlan

AU - Whitfield, Susan

AU - Wong, Wingyui

PY - 2018/10

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