Myocardial infarction in the C57BL/6J mouse - A quantifiable and highly reproducible experimental model

Manuel Salto-Tellez, S.Y. Lim, R.M. El Oakley, T.P.L. Tang, Z.A.M. ALmsherqi, S.K. Lim

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Introduction: The laboratory mouse is a powerful tool in cardiovascular research. In this report, we describe a method for a reproducible mouse myocardial infarction model that would allow subsequent comparative and quantitative studies on molecular and pathophysiological variables. Methods: (A) The distribution of the major coronary arteries including the septal artery in the left ventricle of the C57BL/6J mice (n=20) was mapped by perfusion of latex dye or fluorescent beads through the aorta. (B) The territory of myocardial infarction after the ligation of the most proximal aspect of the left anterior descending (LAD) coronary artery was quantified. (C) The consistency in the histological changes parallel to the infarction at different time points was analyzed. Results: (A) The coronary artery tree of the mouse is different from human and, particularly, in regard to the blood supply of the septum. (B) Contrary to previous belief, the septal coronary artery in the mouse is variable in origin. (C) A constant ligation of the LAD immediately below the left auricular level ensures a statistically significant reproducible infarct size. (D) The ischemic changes can be monitored at a histological level in a way similar to what is described in the human. Conclusion: We illustrate a method for maximal reproducibility of experimental acute myocardial infarction in the mouse model, due to a consistent loss of perfusion in the lower half of the left ventricle. This will allow the study of molecular and physiological variables in a controlled and quantifiable experimental model environment. (C) 2004 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalCardiovascular Pathology
Volume13
Issue number2
DOIs
Publication statusPublished - Mar 2004

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pathology and Forensic Medicine

Fingerprint

Dive into the research topics of 'Myocardial infarction in the C57BL/6J mouse - A quantifiable and highly reproducible experimental model'. Together they form a unique fingerprint.

Cite this