Myosin IIa Promotes Antibody Responses by Regulating B Cell Activation, Acquisition of Antigen, and Proliferation

Robbert Hoogeboom, Elizabeth M. Natkanski, Carla R. Nowosad, Dessislava Malinova, Rajesh P. Menon, Antonio Casal, Pavel Tolar*

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)
1 Downloads (Pure)

Abstract

B cell responses are regulated by antigen acquisition, processing, and presentation to helper T cells. These functions are thought to depend on contractile activity of non-muscle myosin IIa. Here, we show that B cell-specific deletion of the myosin IIa heavy chain reduced the numbers of bone marrow B cell precursors and splenic marginal zone, peritoneal B1b, and germinal center B cells. In addition, myosin IIa-deficient follicular B cells acquired an activated phenotype and were less efficient in chemokinesis and extraction of membrane-presented antigens. Moreover, myosin IIa was indispensable for cytokinesis. Consequently, mice with myosin IIa-deficient B cells harbored reduced serum immunoglobulin levels and did not mount robust antibody responses when immunized. Altogether, these data indicate that myosin IIa is a negative regulator of B cell activation but a positive regulator of antigen acquisition from antigen-presenting cells and that myosin IIa is essential for B cell development, proliferation, and antibody responses. B cell antigen acquisition, processing, and presentation may depend on contractile activity of the actomyosin cytoskeleton. Here, Hoogeboom et al. show that non-muscle myosin IIa positively regulates B cell antigen acquisition from antigen-presenting cells in vivo. In addition, myosin IIa negatively regulates B cell activation and is required for B cell cytokinesis.

Original languageEnglish
Pages (from-to)2342-2353
Number of pages12
JournalCell Reports
Volume23
Issue number8
DOIs
Publication statusPublished - 22 May 2018
Externally publishedYes

Keywords

  • antigen internalization
  • antigen presentation
  • B cell development
  • B cell response
  • B cell signaling
  • cytoskeleton
  • non-muscle myosin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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