NATURAL BIOSILICA AS AN INDEX FOR BONE HEALING: Invited Keynote

Research output: Contribution to conferenceAbstract

Abstract

Diatoms are unicellular microalgae whose cell walls are composed of remarkably uniform, hierarchical micro/nanopatterned, amorphous biosilica that cannot be replicated synthetically. Each species hosts its own unique morphology which is identically replicated generation-to-generation. There are currently estimated to be over 200,000 different diatom species, each with their own unique shape and morphology. This offers a huge array of surface topographies, particle sizes and shapes, each with the same silica precursor. Our research to date has shown that diatom-biosilica is non-cyctotoxic to J774.2 macrophages and hBMSC cells and does not invoke an immunological response or organ toxicity (kidney, spleen and liver) when tested in a murine model. Before testing diatom-biosilica in vivo in an animal fracture model, methods to incorporate the frustules into the defect are being investigated. Two methods have been developed 1) using a bioresorbable hydrogel and 2) 3-D printed polymer-biosilica scaffolds. Both methods have shown promising results with enhanced mechanical properties with the addition of the diatom-biosilica. Work is ongoing to further map and quantify the role of surface topography and chemical cues on cell fate through the systematic in vitro studies of different species of diatom-biosilica.
Original languageEnglish
Publication statusPublished - 25 Sep 2018
Event26th European Orthopaedic Research Society Meeting - NUIG, Galway, Ireland
Duration: 25 Sep 201828 Sep 2018
http://www.eors2018.org

Conference

Conference26th European Orthopaedic Research Society Meeting
Abbreviated titleEORS 18
CountryIreland
CityGalway
Period25/09/201828/09/2018
Internet address

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Diatoms
Bone and Bones
Microalgae
Hydrogel
Particle Size
Silicon Dioxide
Cell Wall
Cues
Polymers
Spleen
Animal Models
Macrophages
Kidney
Liver
Research

Cite this

Walsh, P., & Buchanan, F. (2018). NATURAL BIOSILICA AS AN INDEX FOR BONE HEALING: Invited Keynote. Abstract from 26th European Orthopaedic Research Society Meeting, Galway, Ireland.
Walsh, Pamela ; Buchanan, Fraser. / NATURAL BIOSILICA AS AN INDEX FOR BONE HEALING : Invited Keynote. Abstract from 26th European Orthopaedic Research Society Meeting, Galway, Ireland.
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abstract = "Diatoms are unicellular microalgae whose cell walls are composed of remarkably uniform, hierarchical micro/nanopatterned, amorphous biosilica that cannot be replicated synthetically. Each species hosts its own unique morphology which is identically replicated generation-to-generation. There are currently estimated to be over 200,000 different diatom species, each with their own unique shape and morphology. This offers a huge array of surface topographies, particle sizes and shapes, each with the same silica precursor. Our research to date has shown that diatom-biosilica is non-cyctotoxic to J774.2 macrophages and hBMSC cells and does not invoke an immunological response or organ toxicity (kidney, spleen and liver) when tested in a murine model. Before testing diatom-biosilica in vivo in an animal fracture model, methods to incorporate the frustules into the defect are being investigated. Two methods have been developed 1) using a bioresorbable hydrogel and 2) 3-D printed polymer-biosilica scaffolds. Both methods have shown promising results with enhanced mechanical properties with the addition of the diatom-biosilica. Work is ongoing to further map and quantify the role of surface topography and chemical cues on cell fate through the systematic in vitro studies of different species of diatom-biosilica.",
author = "Pamela Walsh and Fraser Buchanan",
year = "2018",
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day = "25",
language = "English",
note = "26th European Orthopaedic Research Society Meeting, EORS 18 ; Conference date: 25-09-2018 Through 28-09-2018",
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Walsh, P & Buchanan, F 2018, 'NATURAL BIOSILICA AS AN INDEX FOR BONE HEALING: Invited Keynote', 26th European Orthopaedic Research Society Meeting, Galway, Ireland, 25/09/2018 - 28/09/2018.

NATURAL BIOSILICA AS AN INDEX FOR BONE HEALING : Invited Keynote. / Walsh, Pamela; Buchanan, Fraser.

2018. Abstract from 26th European Orthopaedic Research Society Meeting, Galway, Ireland.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - NATURAL BIOSILICA AS AN INDEX FOR BONE HEALING

T2 - Invited Keynote

AU - Walsh, Pamela

AU - Buchanan, Fraser

PY - 2018/9/25

Y1 - 2018/9/25

N2 - Diatoms are unicellular microalgae whose cell walls are composed of remarkably uniform, hierarchical micro/nanopatterned, amorphous biosilica that cannot be replicated synthetically. Each species hosts its own unique morphology which is identically replicated generation-to-generation. There are currently estimated to be over 200,000 different diatom species, each with their own unique shape and morphology. This offers a huge array of surface topographies, particle sizes and shapes, each with the same silica precursor. Our research to date has shown that diatom-biosilica is non-cyctotoxic to J774.2 macrophages and hBMSC cells and does not invoke an immunological response or organ toxicity (kidney, spleen and liver) when tested in a murine model. Before testing diatom-biosilica in vivo in an animal fracture model, methods to incorporate the frustules into the defect are being investigated. Two methods have been developed 1) using a bioresorbable hydrogel and 2) 3-D printed polymer-biosilica scaffolds. Both methods have shown promising results with enhanced mechanical properties with the addition of the diatom-biosilica. Work is ongoing to further map and quantify the role of surface topography and chemical cues on cell fate through the systematic in vitro studies of different species of diatom-biosilica.

AB - Diatoms are unicellular microalgae whose cell walls are composed of remarkably uniform, hierarchical micro/nanopatterned, amorphous biosilica that cannot be replicated synthetically. Each species hosts its own unique morphology which is identically replicated generation-to-generation. There are currently estimated to be over 200,000 different diatom species, each with their own unique shape and morphology. This offers a huge array of surface topographies, particle sizes and shapes, each with the same silica precursor. Our research to date has shown that diatom-biosilica is non-cyctotoxic to J774.2 macrophages and hBMSC cells and does not invoke an immunological response or organ toxicity (kidney, spleen and liver) when tested in a murine model. Before testing diatom-biosilica in vivo in an animal fracture model, methods to incorporate the frustules into the defect are being investigated. Two methods have been developed 1) using a bioresorbable hydrogel and 2) 3-D printed polymer-biosilica scaffolds. Both methods have shown promising results with enhanced mechanical properties with the addition of the diatom-biosilica. Work is ongoing to further map and quantify the role of surface topography and chemical cues on cell fate through the systematic in vitro studies of different species of diatom-biosilica.

UR - https://online.boneandjoint.org.uk/doi/abs/10.1302/1358-992X.2018.14.048

M3 - Abstract

ER -

Walsh P, Buchanan F. NATURAL BIOSILICA AS AN INDEX FOR BONE HEALING: Invited Keynote. 2018. Abstract from 26th European Orthopaedic Research Society Meeting, Galway, Ireland.