Abstract
The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage of Mesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30-39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.
| Original language | English |
|---|---|
| Pages (from-to) | 690-695 |
| Number of pages | 6 |
| Journal | Parasitology Research |
| Volume | 79 |
| Publication status | Published - 1993 |
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Hrckova, G., Halton, D. W., Maule, A., Brennan, G., Shaw, C., & Johnston, C. (1993). NEUROPEPTIDE F-IMMUNOREACTIVITY IN THE TETRATHYRIDIUM OF MESOCESTOIDES-CORTI (CESTODA, CYCLOPHYLLIDEA). Parasitology Research, 79, 690-695.