NEUROPEPTIDE F-IMMUNOREACTIVITY IN THE TETRATHYRIDIUM OF MESOCESTOIDES-CORTI (CESTODA, CYCLOPHYLLIDEA)

G. Hrckova; D W Halton; Aaron Maule; Gerard Brennan; Christopher Shaw; Colin Johnston

NEUROPEPTIDE F-IMMUNOREACTIVITY IN THE TETRATHYRIDIUM OF MESOCESTOIDES-CORTI (CESTODA, CYCLOPHYLLIDEA)

G. Hrckova, D W Halton, Aaron Maule, Gerard Brennan, Christopher Shaw, Colin Johnston

Research output: Contribution to journal › Article › peer-review

Abstract

The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage of Mesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30-39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.

Original language	English
Pages (from-to)	690-695
Number of pages	6
Journal	Parasitology Research
Volume	79
Publication status	Published - 1993

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@article{dbfc871e64d44e43a66d699d76de46b9,

title = "NEUROPEPTIDE F-IMMUNOREACTIVITY IN THE TETRATHYRIDIUM OF MESOCESTOIDES-CORTI (CESTODA, CYCLOPHYLLIDEA)",

abstract = "The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage of Mesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30-39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.",

author = "G. Hrckova and Halton, {D W} and Aaron Maule and Gerard Brennan and Christopher Shaw and Colin Johnston",

year = "1993",

language = "English",

volume = "79",

pages = "690--695",

journal = "Parasitology Research",

issn = "0932-0113",

publisher = "Springer Verlag",

}

TY - JOUR

T1 - NEUROPEPTIDE F-IMMUNOREACTIVITY IN THE TETRATHYRIDIUM OF MESOCESTOIDES-CORTI (CESTODA, CYCLOPHYLLIDEA)

AU - Hrckova, G.

AU - Halton, D W

AU - Maule, Aaron

AU - Brennan, Gerard

AU - Shaw, Christopher

AU - Johnston, Colin

PY - 1993

Y1 - 1993

N2 - The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage of Mesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30-39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.

AB - The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage of Mesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30-39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.

M3 - Article

VL - 79

SP - 690

EP - 695

JO - Parasitology Research

JF - Parasitology Research

SN - 0932-0113

ER -