TY - JOUR
T1 - Nontypable Haemophilus influenzae displays a prevalent surface structure molecular pattern in clinical isolates
AU - Martí-Lliteras, Pau
AU - López-Gómez, Antonio
AU - Mauro, Silvia
AU - Hood, Derek W
AU - Viadas, Cristina
AU - Calatayud, Laura
AU - Morey, Pau
AU - Servin, Alain
AU - Liñares, Josefina
AU - Oliver, Antonio
AU - Bengoechea, José Antonio
AU - Garmendia, Junkal
PY - 2011
Y1 - 2011
N2 - Non-typable Haemophilus influenzae (NTHi) is a gram negative pathogen that causes acute respiratory infections and is associated with the progression of chronic respiratory diseases. Previous studies have established the existence of a remarkable genetic variability among NTHi strains. In this study we show that, in spite of a high level of genetic heterogeneity, NTHi clinical isolates display a prevalent molecular feature, which could confer fitness during infectious processes. A total of 111 non-isogenic NTHi strains from an identical number of patients, isolated in two distinct geographical locations in the same period of time, were used to analyse nine genes encoding bacterial surface molecules, and revealed the existence of one highly prevalent molecular pattern (lgtF+, lic2A+, lic1D+, lic3A+, lic3B+, siaA-, lic2C+, ompP5+, oapA+) displayed by 94.6% of isolates. Such a genetic profile was associated with a higher bacterial resistance to serum mediated killing and enhanced adherence to human respiratory epithelial cells.
AB - Non-typable Haemophilus influenzae (NTHi) is a gram negative pathogen that causes acute respiratory infections and is associated with the progression of chronic respiratory diseases. Previous studies have established the existence of a remarkable genetic variability among NTHi strains. In this study we show that, in spite of a high level of genetic heterogeneity, NTHi clinical isolates display a prevalent molecular feature, which could confer fitness during infectious processes. A total of 111 non-isogenic NTHi strains from an identical number of patients, isolated in two distinct geographical locations in the same period of time, were used to analyse nine genes encoding bacterial surface molecules, and revealed the existence of one highly prevalent molecular pattern (lgtF+, lic2A+, lic1D+, lic3A+, lic3B+, siaA-, lic2C+, ompP5+, oapA+) displayed by 94.6% of isolates. Such a genetic profile was associated with a higher bacterial resistance to serum mediated killing and enhanced adherence to human respiratory epithelial cells.
U2 - 10.1371/journal.pone.0021133
DO - 10.1371/journal.pone.0021133
M3 - Article
C2 - 21698169
SN - 1932-6203
VL - 6
SP - e21133
JO - PloS one
JF - PloS one
IS - 6
ER -