Norovirus contamination in retail oysters from Beijing and Qingdao, China

Jiang Tao; Han Chunhui; Séamus Fanning; Li Nan; Wang Jiahui; Zhang Hongyuan; Zhang Jing; Li Fengqin

doi:10.1016/j.foodcont.2017.11.043

Norovirus contamination in retail oysters from Beijing and Qingdao, China

Jiang Tao, Han Chunhui, Séamus Fanning, Li Nan, Wang Jiahui, Zhang Hongyuan, Zhang Jing, Li Fengqin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The consumption of raw oyster has been linked to numerous foodborne gastroenteritis outbreaks caused by norovirus (NoV) in different countries of the world, including in China. This study investigated the prevalence of NoV and contamination levels in retail oysters taken from markets in Beijing and Qingdao in China. The oysters were collected monthly from seafood markets between September 2015 and September 2016 (13 months) in both cities. The digestive glands of these oysters were dissected and NoV particles were extracted. Viral RNA was detected using a TaqMan-based real time one-step reverse transcription-PCR (qRT-PCR) protocol. Of total 672 oyster samples assessed, 652 were considered to be valid for inclusion in the study. The prevalence of NoV was 20.71% (135/652), and this included 21.48% (29/135) of the samples that were positive for the GI strain alone, 62.96% (85/135) contained the GII strain alone and 15.56% (21/135) were mixed with GI and GII, respectively. A total of 68 NoV-positive samples were quantified by qRT-PCR and values obtained ranged from 3.55 × 10³ to 1.45 × 10⁶ genomic copies per g digestive tissue. The NoV contamination in retail oysters fluctuated with the sampling month and peaks of contamination occurred in February (49.12%) and March (55.36%) 2016, respectively. Considering some of the oysters were consumed as raw or half-cooked in China, as a risk reduction measure these oysters should be well heated prior to consumption. Furthermore, successive and region-extended monitoring in retail oysters for NoV as well as risk communication is recommended.

Original language	English
Pages (from-to)	415-419
Number of pages	5
Journal	Food Control
Volume	86
Early online date	05 Dec 2017
DOIs	https://doi.org/10.1016/j.foodcont.2017.11.043
Publication status	Published - Apr 2018

Bibliographical note

Funding Information:
This work was supported by the Beijing Natural Science Foundation (grant number: 5141002 ).

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

Norovirus oyster contamination realtime reverse transcription-polymerase chain reaction (qRT-PCR)

ASJC Scopus subject areas

Biotechnology
Food Science

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Norovirus contamination in retail oysters from Beijing and Qingdao, China
Copyright 2017 the authors. This is an open access article published under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.
Final published version, 328 KBLicence: CC BY-NC-ND

Cite this

@article{a775612bb7c14497a803ea066e889748,

title = "Norovirus contamination in retail oysters from Beijing and Qingdao, China",

abstract = "The consumption of raw oyster has been linked to numerous foodborne gastroenteritis outbreaks caused by norovirus (NoV) in different countries of the world, including in China. This study investigated the prevalence of NoV and contamination levels in retail oysters taken from markets in Beijing and Qingdao in China. The oysters were collected monthly from seafood markets between September 2015 and September 2016 (13 months) in both cities. The digestive glands of these oysters were dissected and NoV particles were extracted. Viral RNA was detected using a TaqMan-based real time one-step reverse transcription-PCR (qRT-PCR) protocol. Of total 672 oyster samples assessed, 652 were considered to be valid for inclusion in the study. The prevalence of NoV was 20.71% (135/652), and this included 21.48% (29/135) of the samples that were positive for the GI strain alone, 62.96% (85/135) contained the GII strain alone and 15.56% (21/135) were mixed with GI and GII, respectively. A total of 68 NoV-positive samples were quantified by qRT-PCR and values obtained ranged from 3.55 × 103 to 1.45 × 106 genomic copies per g digestive tissue. The NoV contamination in retail oysters fluctuated with the sampling month and peaks of contamination occurred in February (49.12%) and March (55.36%) 2016, respectively. Considering some of the oysters were consumed as raw or half-cooked in China, as a risk reduction measure these oysters should be well heated prior to consumption. Furthermore, successive and region-extended monitoring in retail oysters for NoV as well as risk communication is recommended.",

keywords = "Norovirus oyster contamination realtime reverse transcription-polymerase chain reaction (qRT-PCR)",

author = "Jiang Tao and Han Chunhui and S{\'e}amus Fanning and Li Nan and Wang Jiahui and Zhang Hongyuan and Zhang Jing and Li Fengqin",

note = "Funding Information: This work was supported by the Beijing Natural Science Foundation (grant number: 5141002 ). Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

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doi = "10.1016/j.foodcont.2017.11.043",

language = "English",

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T1 - Norovirus contamination in retail oysters from Beijing and Qingdao, China

AU - Tao, Jiang

AU - Chunhui, Han

AU - Fanning, Séamus

AU - Nan, Li

AU - Jiahui, Wang

AU - Hongyuan, Zhang

AU - Jing, Zhang

AU - Fengqin, Li

PY - 2018/4

Y1 - 2018/4

N2 - The consumption of raw oyster has been linked to numerous foodborne gastroenteritis outbreaks caused by norovirus (NoV) in different countries of the world, including in China. This study investigated the prevalence of NoV and contamination levels in retail oysters taken from markets in Beijing and Qingdao in China. The oysters were collected monthly from seafood markets between September 2015 and September 2016 (13 months) in both cities. The digestive glands of these oysters were dissected and NoV particles were extracted. Viral RNA was detected using a TaqMan-based real time one-step reverse transcription-PCR (qRT-PCR) protocol. Of total 672 oyster samples assessed, 652 were considered to be valid for inclusion in the study. The prevalence of NoV was 20.71% (135/652), and this included 21.48% (29/135) of the samples that were positive for the GI strain alone, 62.96% (85/135) contained the GII strain alone and 15.56% (21/135) were mixed with GI and GII, respectively. A total of 68 NoV-positive samples were quantified by qRT-PCR and values obtained ranged from 3.55 × 103 to 1.45 × 106 genomic copies per g digestive tissue. The NoV contamination in retail oysters fluctuated with the sampling month and peaks of contamination occurred in February (49.12%) and March (55.36%) 2016, respectively. Considering some of the oysters were consumed as raw or half-cooked in China, as a risk reduction measure these oysters should be well heated prior to consumption. Furthermore, successive and region-extended monitoring in retail oysters for NoV as well as risk communication is recommended.

AB - The consumption of raw oyster has been linked to numerous foodborne gastroenteritis outbreaks caused by norovirus (NoV) in different countries of the world, including in China. This study investigated the prevalence of NoV and contamination levels in retail oysters taken from markets in Beijing and Qingdao in China. The oysters were collected monthly from seafood markets between September 2015 and September 2016 (13 months) in both cities. The digestive glands of these oysters were dissected and NoV particles were extracted. Viral RNA was detected using a TaqMan-based real time one-step reverse transcription-PCR (qRT-PCR) protocol. Of total 672 oyster samples assessed, 652 were considered to be valid for inclusion in the study. The prevalence of NoV was 20.71% (135/652), and this included 21.48% (29/135) of the samples that were positive for the GI strain alone, 62.96% (85/135) contained the GII strain alone and 15.56% (21/135) were mixed with GI and GII, respectively. A total of 68 NoV-positive samples were quantified by qRT-PCR and values obtained ranged from 3.55 × 103 to 1.45 × 106 genomic copies per g digestive tissue. The NoV contamination in retail oysters fluctuated with the sampling month and peaks of contamination occurred in February (49.12%) and March (55.36%) 2016, respectively. Considering some of the oysters were consumed as raw or half-cooked in China, as a risk reduction measure these oysters should be well heated prior to consumption. Furthermore, successive and region-extended monitoring in retail oysters for NoV as well as risk communication is recommended.

KW - Norovirus oyster contamination realtime reverse transcription-polymerase chain reaction (qRT-PCR)

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DO - 10.1016/j.foodcont.2017.11.043

M3 - Article

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VL - 86

SP - 415

EP - 419

JO - Food Control

JF - Food Control

SN - 0956-7135

ER -

Norovirus contamination in retail oysters from Beijing and Qingdao, China

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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