Novel Kazal-type Protease inhibitors from the skin secretion of the Splendid leaf frog, Cruziohyla calcarifer

Carolina Proaño Bolaños, Renjie Li, Mei Zhou, Lei Wang, Xinping Xi, Elicio E. Tapia, Luis A. Coloma, Tianbao Chen, Chris Shaw

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Abstract

Protease inhibitors have an important role controlling a variety of biological processes. Here, we employed a peptidomic approach including molecular cloning, tandem mass spectrometry and enzymatic assays to reveal 7 Kazal-type proteinase inhibitors (CCKPs) (18 variants) in the skin secretion of the unexplored frog, Cruziohyla calcarifer. All 18 proteins shared the Kazal pattern (CX(6-7)-CX(6-7)-Y-X(2-3)-CX(2)-C) and 3 disulphide bridges. Based on structural comparative analysis, we deemed trypsin and chymotrypsin inhibitory activity in CCKP-1, 4 and CCKP 2, 5, 7, respectively. These protease inhibitors presumably play a role to control the balance between other functional peptides produced in the amphibian skin secretions.
Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalEuPA Open Proteomics
Volume15
Early online date27 Feb 2017
DOIs
Publication statusPublished - Jun 2017

Keywords

  • Kazal-type proteinase inhibitors; frog skin secretion; peptidomic; molecular cloning; tandem mass spectrometry; Cruziohyla calcarifer

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