Abstract
Studies into the cell nucleus' incorporation of gold nanoparticles (AuNPs) are often limited by ambiguities arising from conventional imaging techniques. Indeed, it is suggested that to date there is no unambiguous imaging evidence for such uptake in whole cells, particularly at the single nanoparticle level. This shortcoming in understanding exists despite the nucleus being the most important subcellular compartment in eukaryotes and gold being the most commonly used metal nanoparticle in medical applications. Here, dual-angle X-ray flouresence is used to show individually resolved nanoparticles within the cell nucleus, finding them to be well separated and 79% of the intranuclear population to be monodispersed. These findings have important implications for nanomedicine, illustrated here through a specific exemplar of the predicted enhancement of radiation effects arising from the observed AuNPs, finding intranuclear dose enhancements spanning nearly five orders of magnitude.
Original language | English |
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Article number | 1900140 |
Journal | Particle and Particle Systems Characterization |
DOIs | |
Publication status | Published - 01 Jan 2019 |
Keywords
- gold nanoparticles
- nanomedicine
- nuclear uptake
- X-ray fluorescence
- X-ray microscopy
ASJC Scopus subject areas
- General Chemistry
- General Materials Science
- Condensed Matter Physics
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Dataset for "Gold nanoparticle cellular uptake and its implications for cancer therapy"
McCulloch, A. (Creator), Queen's University Belfast, 02 Dec 2020
DOI: 10.17034/989e4cf1-9c9b-4d6f-a4fc-ebbbfc4fb58c
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Student theses
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Enhancing the radio sensitivity of prostate cancer using RALA Gold nano-complexes
Bennie, L. (Author), McCarthy, H. (Supervisor) & Coulter, J. (Supervisor), Jul 2020Student thesis: Doctoral Thesis › Doctor of Philosophy
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Gold nanoparticle cellular uptake and its implications for cancer therapy
McCulloch, A. (Author), Dromey, B. (Supervisor) & Prise, K. (Supervisor), Jul 2021Student thesis: Doctoral Thesis › Doctor of Philosophy
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