Organophosphonate metabolism by a moderately halophilic bacterial isolate

Velma E A Hayes; Nigel G. Ternan; Geoffrey McMullan

doi:10.1016/S0378-1097(00)00136-1

Organophosphonate metabolism by a moderately halophilic bacterial isolate

Velma E A Hayes, Nigel G. Ternan^*, Geoffrey McMullan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethylphosphonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity. Copyright (C) 2000 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	171-175
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	186
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(00)00136-1
Publication status	Published - 05 May 2000
Externally published	Yes

Keywords

Biodegradation
C-P lyase
Halophilic
Organophosphonate
Pho regulon

ASJC Scopus subject areas

Genetics
Molecular Biology
Applied Microbiology and Biotechnology
Microbiology

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10.1016/S0378-1097(00)00136-1

Cite this

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title = "Organophosphonate metabolism by a moderately halophilic bacterial isolate",

abstract = "A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethylphosphonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity. Copyright (C) 2000 Federation of European Microbiological Societies.",

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AU - Hayes, Velma E A

AU - Ternan, Nigel G.

AU - McMullan, Geoffrey

PY - 2000/5/5

Y1 - 2000/5/5

N2 - A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethylphosphonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity. Copyright (C) 2000 Federation of European Microbiological Societies.

AB - A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethylphosphonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity. Copyright (C) 2000 Federation of European Microbiological Societies.

KW - Biodegradation

KW - C-P lyase

KW - Halophilic

KW - Organophosphonate

KW - Pho regulon

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SN - 0378-1097

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IS - 2

ER -

Organophosphonate metabolism by a moderately halophilic bacterial isolate

Abstract

Keywords

ASJC Scopus subject areas

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