Overcoming Equilibrium Issues with Carbonyl Reductase Enzymes

S.J. Calvin, David Mangan, Iain Miskelly, Thomas S. Moody, Paul J. Stevenson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We report herein the screening, optimisation and scale up to 100 g of a bioreduction process that employs an in situ product removal (ISPR) technique to overcome the inherent equilibrium problem associated with the coupled-substrate approach to biocatalytic carbonyl reduction. This technique allowed the valuable chiral alcohol, (S)-2-bromo-2-cyclohexen-1-ol, to be isolated in 88% yield and 99.8% ee without the need for further purification, validating the general applicability of this experimental setup.
Original languageEnglish
Pages (from-to)82-86
Number of pages5
JournalOrganic Process Research & Development
Volume16
Issue number1
Early online date04 Nov 2011
DOIs
Publication statusPublished - 20 Jan 2012

ASJC Scopus subject areas

  • Organic Chemistry
  • Physical and Theoretical Chemistry

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