Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide

Pradel Tonda-Mikiela; Aurélien Habrioux; Susan Boland; Karine Servat; Sophie Tingry; Paul Kavanagh; Teko W. Napporn; Donal Leech; Boniface B. Kokoh

doi:10.1007/s12678-011-0062-1

Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide

Pradel Tonda-Mikiela, Aurélien Habrioux, Susan Boland, Karine Servat, Sophie Tingry, Paul Kavanagh, Teko W. Napporn, Donal Leech, Boniface B. Kokoh

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

This paper reports on the electroreduction of oxygen catalyzed by bilirubin oxidase (BOD) without H ₂O ₂ production. The enzyme was immobilized in a Nafion® matrix in the presence of either a commercial mediator, the 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), or a synthesized osmium redox complex to increase the kinetically slow bio-electroreaction. The use of a rotating ring-disk electrode system demonstrated for the first time the absence of hydrogen peroxide production during the oxygen reduction reaction at the active BOD centers mediated by ABTS. These results were compared with those obtained on Pt-based catalyst. We showed that BOD has the advantage to reduce O ₂ at a smaller overpotential value (100 mV) than Pt (260 mV).

Original language	English
Pages (from-to)	268-272
Number of pages	5
Journal	Electrocatalysis
Volume	2
Issue number	4
DOIs	https://doi.org/10.1007/s12678-011-0062-1
Publication status	Published - Dec 2011
Externally published	Yes

Keywords

Bilirubin oxidase
Osmium complex
Oxygen reduction

ASJC Scopus subject areas

Electrochemistry

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10.1007/s12678-011-0062-1

Cite this

@article{ec7235233e304cd2843e51ada74ab050,

title = "Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide",

abstract = "This paper reports on the electroreduction of oxygen catalyzed by bilirubin oxidase (BOD) without H 2O 2 production. The enzyme was immobilized in a Nafion{\textregistered} matrix in the presence of either a commercial mediator, the 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), or a synthesized osmium redox complex to increase the kinetically slow bio-electroreaction. The use of a rotating ring-disk electrode system demonstrated for the first time the absence of hydrogen peroxide production during the oxygen reduction reaction at the active BOD centers mediated by ABTS. These results were compared with those obtained on Pt-based catalyst. We showed that BOD has the advantage to reduce O 2 at a smaller overpotential value (100 mV) than Pt (260 mV).",

keywords = "Bilirubin oxidase, Osmium complex, Oxygen reduction",

author = "Pradel Tonda-Mikiela and Aur{\'e}lien Habrioux and Susan Boland and Karine Servat and Sophie Tingry and Paul Kavanagh and Napporn, {Teko W.} and Donal Leech and Kokoh, {Boniface B.}",

year = "2011",

month = dec,

doi = "10.1007/s12678-011-0062-1",

language = "English",

volume = "2",

pages = "268--272",

journal = "Electrocatalysis",

issn = "1868-2529",

publisher = "Springer Publishing Company",

number = "4",

}

Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide. / Tonda-Mikiela, Pradel; Habrioux, Aurélien; Boland, Susan; Servat, Karine; Tingry, Sophie; Kavanagh, Paul; Napporn, Teko W.; Leech, Donal; Kokoh, Boniface B.

In: Electrocatalysis, Vol. 2, No. 4, 12.2011, p. 268-272.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide

AU - Tonda-Mikiela, Pradel

AU - Habrioux, Aurélien

AU - Boland, Susan

AU - Servat, Karine

AU - Tingry, Sophie

AU - Kavanagh, Paul

AU - Napporn, Teko W.

AU - Leech, Donal

AU - Kokoh, Boniface B.

PY - 2011/12

Y1 - 2011/12

N2 - This paper reports on the electroreduction of oxygen catalyzed by bilirubin oxidase (BOD) without H 2O 2 production. The enzyme was immobilized in a Nafion® matrix in the presence of either a commercial mediator, the 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), or a synthesized osmium redox complex to increase the kinetically slow bio-electroreaction. The use of a rotating ring-disk electrode system demonstrated for the first time the absence of hydrogen peroxide production during the oxygen reduction reaction at the active BOD centers mediated by ABTS. These results were compared with those obtained on Pt-based catalyst. We showed that BOD has the advantage to reduce O 2 at a smaller overpotential value (100 mV) than Pt (260 mV).

AB - This paper reports on the electroreduction of oxygen catalyzed by bilirubin oxidase (BOD) without H 2O 2 production. The enzyme was immobilized in a Nafion® matrix in the presence of either a commercial mediator, the 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), or a synthesized osmium redox complex to increase the kinetically slow bio-electroreaction. The use of a rotating ring-disk electrode system demonstrated for the first time the absence of hydrogen peroxide production during the oxygen reduction reaction at the active BOD centers mediated by ABTS. These results were compared with those obtained on Pt-based catalyst. We showed that BOD has the advantage to reduce O 2 at a smaller overpotential value (100 mV) than Pt (260 mV).

KW - Bilirubin oxidase

KW - Osmium complex

KW - Oxygen reduction

UR - http://www.scopus.com/inward/record.url?scp=84864450960&partnerID=8YFLogxK

U2 - 10.1007/s12678-011-0062-1

DO - 10.1007/s12678-011-0062-1

M3 - Article

AN - SCOPUS:84864450960

VL - 2

SP - 268

EP - 272

JO - Electrocatalysis

JF - Electrocatalysis

SN - 1868-2529

IS - 4

ER -

Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide

Abstract

Keywords

ASJC Scopus subject areas

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