Oxygen enhancement ratios of cancer cells after exposure to intensity modulated x-ray fields: DNA damage and cell survival

Yusuke Matsuya, Stephen J. McMahon, Karl T. Butterworth, Shingo Naijo, Isshi Nara, Yoshie Yachi, Ryo Saga, Masayori Ishikawa, Tatsuhiko Sato, Hiroyuki Date, Kevin M. Prise

Research output: Contribution to journalArticlepeer-review

Abstract

Hypoxic cancer cells within solid tumours show radio-resistance, leading to malignant progression in fractionated radiotherapy. When prescribing dose to tumours under heterogeneous oxygen pressure with intensity-modulated radiation fields, intercellular signalling could have an impact on radiosensitivity between in-field and out-of-field (OF) cells. However, the impact of hypoxia on radiosensitivity under modulated radiation intensity remains to be fully clarified. Here, we investigate the impact of hypoxia on in-field and OF radio-sensitivities using two types of cancer cells, DU145 and H1299. Using a nBIONIX hypoxic culture kit and a shielding technique to irradiate 50% of a cell culture flask, oxygen enhancement ratios for double-strand breaks (DSB) and cell death endpoints were determined. These in vitro measurements indicate that hypoxia impacts OF cells, although the hypoxic impacts on OF cells for cell survival were dose-dependent and smaller compared to those for in-field and uniformly irradiated cells. These decreased radio-sensitivities of OF cells were shown as a consistent tendency for both DSB and cell death endpoints, suggesting that radiation-induced intercellular communication is of importance in advanced radiotherapy dose-distributions such as with intensity-modulated radiotherapy.

Original languageEnglish
Article number075014
Number of pages12
JournalPhysics in Medicine and Biology
Volume66
Issue number7
DOIs
Publication statusPublished - 06 Apr 2021

Bibliographical note

Publisher Copyright:
© 2021 Institute of Physics Publishing. All rights reserved.

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Keywords

  • Cell survival
  • DNA damage
  • Intensity modulated radiation fields
  • Oxygen enhancement ratio

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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