Pan-genome analysis of the emerging foodborne pathogen Cronobacter spp. suggests a species-level bidirectional divergence driven by niche adaptation

Christopher J. Grim, Michael L. Kotewicz, Karen A. Power, Gopal Gopinath, Augusto A. Franco, Karen G. Jarvis, Qiong Q. Yan, Scott A. Jackson, Venugopal Sathyamoorthy, Lan Hu, Franco Pagotto, Carol Iversen, Angelika Lehner, Roger Stephan, Séamus Fanning, Ben D. Tall*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    55 Citations (Scopus)
    222 Downloads (Pure)

    Abstract

    Background: Members of the genus Cronobacter are causes of rare but severe illness in neonates and preterm infants following the ingestion of contaminated infant formula. Seven species have been described and two of the species genomes were subsequently published. In this study, we performed comparative genomics on eight strains of Cronobacter, including six that we sequenced (representing six of the seven species) and two previously published, closed genomes.

    Results: We identified and characterized the features associated with the core and pan genome of the genus Cronobacter in an attempt to understand the evolution of these bacteria and the genetic content of each species. We identified 84 genomic regions that are present in two or more Cronobacter genomes, along with 45 unique genomic regions. Many potentially horizontally transferred genes, such as lysogenic prophages, were also identified. Most notable among these were several type six secretion system gene clusters, transposons that carried tellurium, copper and/or silver resistance genes, and a novel integrative conjugative element.

    Conclusions: Cronobacter have diverged into two clusters, one consisting of C. dublinensis and C. muytjensii (Cdub-Cmuy) and the other comprised of C. sakazakii, C. malonaticus, C. universalis, and C. turicensis, (Csak-Cmal-Cuni-Ctur) from the most recent common ancestral species. While several genetic determinants for plant-association and human virulence could be found in the core genome of Cronobacter, the four Cdub-Cmuy clade genomes contained several accessory genomic regions important for survival in a plant-associated environmental niche, while the Csak-Cmal-Cuni-Ctur clade genomes harbored numerous virulence-related genetic traits.

    Original languageEnglish
    Article number366
    Number of pages16
    JournalBMC Genomics
    Volume14
    DOIs
    Publication statusPublished - 31 May 2013

    Keywords

    • INFANT MILK FORMULA
    • ENTEROBACTER-SAKAZAKII
    • THERMAL-RESISTANCE
    • VIBRIO-CHOLERAE
    • V. CHOLERAE
    • RPOB GENE
    • SEQUENCE
    • FOOD
    • IDENTIFICATION
    • PERSISTENCE

    ASJC Scopus subject areas

    • Biotechnology
    • Genetics

    Fingerprint

    Dive into the research topics of 'Pan-genome analysis of the emerging foodborne pathogen <i>Cronobacter</i> spp. suggests a species-level bidirectional divergence driven by niche adaptation'. Together they form a unique fingerprint.

    Cite this