Phenotypic changes contributing to Enterobacter gergoviae biocide resistance

M. Périamé, N. Philippe, O. Condell, S. Fanning, J. M. Pagès, A. Davin-Regli*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Enterobacter gergoviae is a recurrent contaminant of cosmetic and hygiene products. To understand how this bacterium adapts to biocides, we studied Ent. gergoviae CIP 76.01 and its triclosan and Methylisothiazolinone-chloromethylisothiazolinone (MIT-CMIT) tolerant isogenic mutants. They were compared with others also isolated from contaminated cosmetics. Phenotypic differences were noted and these included changes in the bacterial envelope and flagella along with differences in motility, and biofilm growth rates. Triclosan and MIT-CMIT derivatives expressed flagella and other MIT-CMIT derivatives exhibited some external appendages. Those bacteria expressing a high-level minimal inhibitory concentration to MIT-CMIT, expressed a strong biofilm formation. No differential phenotypes were noted for carbon source utilisation. Enterobacter gergoviae demonstrated a diverse response to both of these preservatives contained in cosmetic preparations, depending on their concentrations. Interestingly, this adaptive response is associated with modifications of filament structure-related proteins contributing to increase the organism motility and the production of biofilm.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalLetters in Applied Microbiology
Volume61
Issue number2
Early online date29 May 2015
DOIs
Publication statusPublished - 01 Aug 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 The Society for Applied Microbiology.

Keywords

  • Biofilm
  • Cosmetics
  • Enterobacter gergoviae
  • Fimbriae
  • Flagellum
  • Preservatives
  • Triclosan

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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