Phosphonoacetate biosynthesis: In vitro detection of a novel NADP(+)-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of a marine Roseobacter

N.A. Cooley, A.N. Kulakova, J.F. Villarreal-Chiu, J.A. Gilbert, John McGrath, John Quinn

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A novel phosphonoacetaldehyde-oxidizing activity was detected in cell-extracts of the marine bacterium Roseovarius nubinhibens ISM grown on 2-aminoethylphosphonic acid (2-AEP; ciliatine). Extracts also contained 2-AEP transaminase and phosphonoacetate hydrolase activities. These findings indicate the existence of a biological route from 2-AEP via phosphonoacetaldehyde for the production of phosphonoacetate, which has not previously been shown to be a natural product. The three enzymes appear to constitute a previously-unreported pathway for the mineralization of 2-AEP which is a potentially important source of phosphorus in the nutrient-stressed marine environment.
Original languageEnglish
Pages (from-to)335-340
Number of pages6
JournalMicrobiology
Volume80
Issue number3
DOIs
Publication statusPublished - Jun 2011

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Phosphonoacetate biosynthesis: In vitro detection of a novel NADP(+)-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of a marine Roseobacter'. Together they form a unique fingerprint.

Cite this