Photoelectrochemical Detection of β-amyloid Peptides by a TiO2 Nanobrush Biosensor

Yu Jen Lu; Agnes Purwidyantri; Hui Ling Liu; Le Wen Wang; Cheng Ye Shih; Dorota G. Pijanowska; Chia Ming Yang

doi:10.1109/JSEN.2020.2976561

Photoelectrochemical Detection of β-amyloid Peptides by a TiO₂ Nanobrush Biosensor

Yu Jen Lu, Agnes Purwidyantri, Hui Ling Liu, Le Wen Wang, Cheng Ye Shih, Dorota G. Pijanowska, Chia Ming Yang^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

95 Downloads (Pure)

Abstract

A simple, facile and cost-effective nanostructuring technique is proposed to construct a photoelectrochemical (PEC) sensor via the solution-based hydrothermal growth of a TiO₂ nanobrush (TiO₂ NB). It is demonstrated that the control of the TiO₂ seed solution ratio, process temperature and duration significantly contributed to the final morphological characteristics of the rutile TiO₂ NB, as confirmed by the X-ray diffraction (XRD) analysis. By carrying out a simple and inexpensive fabrication involving no additional surface modification materials, the proposed TiO₂ NB enabled the enhancement of the surface area by up to 162% in comparison with its actual geometric area. The photoactivity achieved by applying UV-range light was drastically improved, and photocurrents could potentially be utilized to enhance the redox activity on the interface. The system was used to detect $\beta $ -amyloid ( $\text{A}\beta $ )_1-28 peptides, one of the most crucial biomarkers of patients with Alzheimer's disease (AD), and demonstrated an excellent biocompatibility caused by the straightforward self-assembled monolayers (SAMs) modified on its surface. For the detection of a wide range of $\text{A}\beta _{\text {1-28}}$ peptides, the constructed TiO₂ NB photoelectrochemical (PEC) sensor exhibited a great sensitivity of $114.8\mu $ A/(ng^. mL^-1) and limit of detection (LoD) of 26.3 ng.mL^-1, facilitating a simple, label-free, rapid, sensitive and noninvasive method to overcome the limitations of conventional techniques used for AD diagnosis.

Original language	English
Article number	9018238
Pages (from-to)	6248-6255
Number of pages	8
Journal	IEEE Sensors Journal
Volume	20
Issue number	12
DOIs	https://doi.org/10.1109/JSEN.2020.2976561
Publication status	Published - 15 Jun 2020

Bibliographical note

Funding Information:
Manuscript received November 12, 2019; revised February 15, 2020; accepted February 20, 2020. Date of publication February 28, 2020; date of current version May 15, 2020. This work was supported in part by the Chang Gung Memorial Hospital (CGMH), Taiwan, under Contract CMRPD2G0202, Contract CMRPD2K0021, and Contract CMRPG3F1743 and in part by the Ministry of Science and Technology (MOST), Taiwan, under Contract MOST 108-2221-E-182-060-MY3 and Contract 108-2628-E-182-002-MY3. The associate editor coordinating the review of this article and approving it for publication was Dr. Gymama Slaughter. (Corresponding author: Chia-Ming Yang.) Yu-Jen Lu is with the Department of Neurosurgery, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, and also with the College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.

Publisher Copyright:
© 2001-2012 IEEE.

Keywords

Alzheimer's disease (AD)
hydrothermal
photoelectrochemical (PEC) sensor
surface area
TiO₂ nanobrush (TiO₂ NB)
β-amyloid (Aβ)

ASJC Scopus subject areas

Instrumentation
Electrical and Electronic Engineering

Access to Document

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Photoelectrochemical Detection of β-amyloid Peptides by a TiO2 Nanobrush Biosensor
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Cite this

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title = "Photoelectrochemical Detection of β-amyloid Peptides by a TiO2 Nanobrush Biosensor",

abstract = "A simple, facile and cost-effective nanostructuring technique is proposed to construct a photoelectrochemical (PEC) sensor via the solution-based hydrothermal growth of a TiO2 nanobrush (TiO2 NB). It is demonstrated that the control of the TiO2 seed solution ratio, process temperature and duration significantly contributed to the final morphological characteristics of the rutile TiO2 NB, as confirmed by the X-ray diffraction (XRD) analysis. By carrying out a simple and inexpensive fabrication involving no additional surface modification materials, the proposed TiO2 NB enabled the enhancement of the surface area by up to 162% in comparison with its actual geometric area. The photoactivity achieved by applying UV-range light was drastically improved, and photocurrents could potentially be utilized to enhance the redox activity on the interface. The system was used to detect $\beta $ -amyloid ( $\text{A}\beta $ )1-28 peptides, one of the most crucial biomarkers of patients with Alzheimer's disease (AD), and demonstrated an excellent biocompatibility caused by the straightforward self-assembled monolayers (SAMs) modified on its surface. For the detection of a wide range of $\text{A}\beta _{\text {1-28}}$ peptides, the constructed TiO2 NB photoelectrochemical (PEC) sensor exhibited a great sensitivity of $114.8\mu $ A/(ng. mL-1) and limit of detection (LoD) of 26.3 ng.mL-1, facilitating a simple, label-free, rapid, sensitive and noninvasive method to overcome the limitations of conventional techniques used for AD diagnosis.",

keywords = "Alzheimer's disease (AD), hydrothermal, photoelectrochemical (PEC) sensor, surface area, TiO₂ nanobrush (TiO₂ NB), β-amyloid (Aβ)",

author = "Lu, {Yu Jen} and Agnes Purwidyantri and Liu, {Hui Ling} and Wang, {Le Wen} and Shih, {Cheng Ye} and Pijanowska, {Dorota G.} and Yang, {Chia Ming}",

note = "Funding Information: Manuscript received November 12, 2019; revised February 15, 2020; accepted February 20, 2020. Date of publication February 28, 2020; date of current version May 15, 2020. This work was supported in part by the Chang Gung Memorial Hospital (CGMH), Taiwan, under Contract CMRPD2G0202, Contract CMRPD2K0021, and Contract CMRPG3F1743 and in part by the Ministry of Science and Technology (MOST), Taiwan, under Contract MOST 108-2221-E-182-060-MY3 and Contract 108-2628-E-182-002-MY3. The associate editor coordinating the review of this article and approving it for publication was Dr. Gymama Slaughter. (Corresponding author: Chia-Ming Yang.) Yu-Jen Lu is with the Department of Neurosurgery, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, and also with the College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. Publisher Copyright: {\textcopyright} 2001-2012 IEEE.",

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AU - Lu, Yu Jen

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AU - Shih, Cheng Ye

AU - Pijanowska, Dorota G.

AU - Yang, Chia Ming

N1 - Funding Information: Manuscript received November 12, 2019; revised February 15, 2020; accepted February 20, 2020. Date of publication February 28, 2020; date of current version May 15, 2020. This work was supported in part by the Chang Gung Memorial Hospital (CGMH), Taiwan, under Contract CMRPD2G0202, Contract CMRPD2K0021, and Contract CMRPG3F1743 and in part by the Ministry of Science and Technology (MOST), Taiwan, under Contract MOST 108-2221-E-182-060-MY3 and Contract 108-2628-E-182-002-MY3. The associate editor coordinating the review of this article and approving it for publication was Dr. Gymama Slaughter. (Corresponding author: Chia-Ming Yang.) Yu-Jen Lu is with the Department of Neurosurgery, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, and also with the College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. Publisher Copyright: © 2001-2012 IEEE.

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N2 - A simple, facile and cost-effective nanostructuring technique is proposed to construct a photoelectrochemical (PEC) sensor via the solution-based hydrothermal growth of a TiO2 nanobrush (TiO2 NB). It is demonstrated that the control of the TiO2 seed solution ratio, process temperature and duration significantly contributed to the final morphological characteristics of the rutile TiO2 NB, as confirmed by the X-ray diffraction (XRD) analysis. By carrying out a simple and inexpensive fabrication involving no additional surface modification materials, the proposed TiO2 NB enabled the enhancement of the surface area by up to 162% in comparison with its actual geometric area. The photoactivity achieved by applying UV-range light was drastically improved, and photocurrents could potentially be utilized to enhance the redox activity on the interface. The system was used to detect $\beta $ -amyloid ( $\text{A}\beta $ )1-28 peptides, one of the most crucial biomarkers of patients with Alzheimer's disease (AD), and demonstrated an excellent biocompatibility caused by the straightforward self-assembled monolayers (SAMs) modified on its surface. For the detection of a wide range of $\text{A}\beta _{\text {1-28}}$ peptides, the constructed TiO2 NB photoelectrochemical (PEC) sensor exhibited a great sensitivity of $114.8\mu $ A/(ng. mL-1) and limit of detection (LoD) of 26.3 ng.mL-1, facilitating a simple, label-free, rapid, sensitive and noninvasive method to overcome the limitations of conventional techniques used for AD diagnosis.

AB - A simple, facile and cost-effective nanostructuring technique is proposed to construct a photoelectrochemical (PEC) sensor via the solution-based hydrothermal growth of a TiO2 nanobrush (TiO2 NB). It is demonstrated that the control of the TiO2 seed solution ratio, process temperature and duration significantly contributed to the final morphological characteristics of the rutile TiO2 NB, as confirmed by the X-ray diffraction (XRD) analysis. By carrying out a simple and inexpensive fabrication involving no additional surface modification materials, the proposed TiO2 NB enabled the enhancement of the surface area by up to 162% in comparison with its actual geometric area. The photoactivity achieved by applying UV-range light was drastically improved, and photocurrents could potentially be utilized to enhance the redox activity on the interface. The system was used to detect $\beta $ -amyloid ( $\text{A}\beta $ )1-28 peptides, one of the most crucial biomarkers of patients with Alzheimer's disease (AD), and demonstrated an excellent biocompatibility caused by the straightforward self-assembled monolayers (SAMs) modified on its surface. For the detection of a wide range of $\text{A}\beta _{\text {1-28}}$ peptides, the constructed TiO2 NB photoelectrochemical (PEC) sensor exhibited a great sensitivity of $114.8\mu $ A/(ng. mL-1) and limit of detection (LoD) of 26.3 ng.mL-1, facilitating a simple, label-free, rapid, sensitive and noninvasive method to overcome the limitations of conventional techniques used for AD diagnosis.

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KW - β-amyloid (Aβ)

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