Plasmid DNA damage following exposure to atmospheric pressure non-thermal plasma: kinetics and influence of oxygen admixture

Mahmoud Y Alkawareek, Nid'a H. Alshraiedeh, Sarah Higginbotham, Padrig B. Flynn, Qais T. Algwari, Sean P. Gorman, William G. Graham, Brendan F Gilmore

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The nature and kinetics of plasmid DNA damage after DNA exposure to a kHz-driven atmospheric pressure nonthermal plasma jet has been investigated. Both single-strand break (SSB) and double-strand break (DSB) processes are reported here. While SSB had a higher rate constant, DSB is recognized to be more significant in living systems, often resulting in loss of viability. In a helium-operated plasma jet, adding oxygen to the feed gas resulted in higher rates of DNA DSB, which increased linearly with increasing oxygen content, up to an optimum level of 0.75% oxygen, after which the DSB rate decreased slightly, indicating an essential role for reactive oxygen species in the rapid degradation of DNA.
Original languageEnglish
Pages (from-to)211-219
Number of pages9
JournalPlasma Medicine
Volume4
Issue number1-4
DOIs
Publication statusPublished - 2015

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