Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial

Lauren Brady; Brian Hayes; Gráinne Sheill; Anne-Marie Baird; Emer Guinan; Bryan Stanfill; Tatjana Vlajnic; Orla Casey; Verena Murphy; John Greene; Emma H Allott; Juliette Hussey; Fidelma Cahill; Mieke Van Hemelrijck; Nicola Peat; Lorelei Mucci; Moya Cunningham; Liam Grogan; Thomas Lynch; Rustom P Manecksha; John McCaffrey; Dearbhaile O'Donnell; Orla Sheils; John O'Leary; Sarah Rudman; Ray McDermott; Stephen Finn

doi:10.1371/journal.pone.0243928

Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial

Lauren Brady, Brian Hayes, Gráinne Sheill, Anne-Marie Baird, Emer Guinan, Bryan Stanfill, Tatjana Vlajnic, Orla Casey, Verena Murphy, John Greene, Emma H Allott, Juliette Hussey, Fidelma Cahill, Mieke Van Hemelrijck, Nicola Peat, Lorelei Mucci, Moya Cunningham, Liam Grogan, Thomas Lynch, Rustom P ManeckshaJohn McCaffrey, Dearbhaile O'Donnell, Orla Sheils, John O'Leary, Sarah Rudman, Ray McDermott, Stephen Finn

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Abstract

BACKGROUND: Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine the effectiveness of a structured exercise programme in modulating levels of CTCs and platelet cloaking in patients with metastatic prostate cancer.

METHODS: Participants (n = 61) were randomised into either standard care (control) or exercise arms. Whole blood was collected for all participants at baseline (T0), three months (T3) and six months (T6), and analysed for the presence of CTCs, CTC clusters and platelet cloaking. CTC data was correlated with clinico-pathological information.

RESULTS: Changes in CTC number were observed within group over time, however no significant difference in CTC number was observed between groups over time. Platelet cloaking was identified in 29.5% of participants. A positive correlation between CTC number and white cell count (WCC) was observed (p = 0.0001), in addition to a positive relationship between CTC clusters and PSA levels (p = 0.0393).

CONCLUSION: The presence of platelet cloaking has been observed in this patient population for the first time, in addition to a significant correlation between CTC number and WCC.

TRIAL REGISTRATION: ClincalTrials.gov identifier NCT02453139.

Original language	English
Article number	e0243928
Number of pages	15
Journal	PLoS ONE
Volume	15
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0243928
Publication status	Published - 18 Dec 2020

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Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial
Copyright 2020 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 1.31 MBLicence: CC BY

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Brady, L., Hayes, B., Sheill, G., Baird, A.-M., Guinan, E., Stanfill, B., Vlajnic, T., Casey, O., Murphy, V., Greene, J., Allott, E. H., Hussey, J., Cahill, F., Van Hemelrijck, M., Peat, N., Mucci, L., Cunningham, M., Grogan, L., Lynch, T., ... Finn, S. (2020). Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial. PLoS ONE, 15(12), Article e0243928. https://doi.org/10.1371/journal.pone.0243928

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title = "Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial",

abstract = "BACKGROUND: Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine the effectiveness of a structured exercise programme in modulating levels of CTCs and platelet cloaking in patients with metastatic prostate cancer.METHODS: Participants (n = 61) were randomised into either standard care (control) or exercise arms. Whole blood was collected for all participants at baseline (T0), three months (T3) and six months (T6), and analysed for the presence of CTCs, CTC clusters and platelet cloaking. CTC data was correlated with clinico-pathological information.RESULTS: Changes in CTC number were observed within group over time, however no significant difference in CTC number was observed between groups over time. Platelet cloaking was identified in 29.5% of participants. A positive correlation between CTC number and white cell count (WCC) was observed (p = 0.0001), in addition to a positive relationship between CTC clusters and PSA levels (p = 0.0393).CONCLUSION: The presence of platelet cloaking has been observed in this patient population for the first time, in addition to a significant correlation between CTC number and WCC.TRIAL REGISTRATION: ClincalTrials.gov identifier NCT02453139.",

author = "Lauren Brady and Brian Hayes and Gr{\'a}inne Sheill and Anne-Marie Baird and Emer Guinan and Bryan Stanfill and Tatjana Vlajnic and Orla Casey and Verena Murphy and John Greene and Allott, {Emma H} and Juliette Hussey and Fidelma Cahill and {Van Hemelrijck}, Mieke and Nicola Peat and Lorelei Mucci and Moya Cunningham and Liam Grogan and Thomas Lynch and Manecksha, {Rustom P} and John McCaffrey and Dearbhaile O'Donnell and Orla Sheils and John O'Leary and Sarah Rudman and Ray McDermott and Stephen Finn",

year = "2020",

month = dec,

day = "18",

doi = "10.1371/journal.pone.0243928",

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Brady, L, Hayes, B, Sheill, G, Baird, A-M, Guinan, E, Stanfill, B, Vlajnic, T, Casey, O, Murphy, V, Greene, J, Allott, EH, Hussey, J, Cahill, F, Van Hemelrijck, M, Peat, N, Mucci, L, Cunningham, M, Grogan, L, Lynch, T, Manecksha, RP, McCaffrey, J, O'Donnell, D, Sheils, O, O'Leary, J, Rudman, S, McDermott, R & Finn, S 2020, 'Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial', PLoS ONE, vol. 15, no. 12, e0243928. https://doi.org/10.1371/journal.pone.0243928

TY - JOUR

T1 - Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial

AU - Brady, Lauren

AU - Hayes, Brian

AU - Sheill, Gráinne

AU - Baird, Anne-Marie

AU - Guinan, Emer

AU - Stanfill, Bryan

AU - Vlajnic, Tatjana

AU - Casey, Orla

AU - Murphy, Verena

AU - Greene, John

AU - Allott, Emma H

AU - Hussey, Juliette

AU - Cahill, Fidelma

AU - Van Hemelrijck, Mieke

AU - Peat, Nicola

AU - Mucci, Lorelei

AU - Cunningham, Moya

AU - Grogan, Liam

AU - Lynch, Thomas

AU - Manecksha, Rustom P

AU - McCaffrey, John

AU - O'Donnell, Dearbhaile

AU - Sheils, Orla

AU - O'Leary, John

AU - Rudman, Sarah

AU - McDermott, Ray

AU - Finn, Stephen

PY - 2020/12/18

Y1 - 2020/12/18

N2 - BACKGROUND: Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine the effectiveness of a structured exercise programme in modulating levels of CTCs and platelet cloaking in patients with metastatic prostate cancer.METHODS: Participants (n = 61) were randomised into either standard care (control) or exercise arms. Whole blood was collected for all participants at baseline (T0), three months (T3) and six months (T6), and analysed for the presence of CTCs, CTC clusters and platelet cloaking. CTC data was correlated with clinico-pathological information.RESULTS: Changes in CTC number were observed within group over time, however no significant difference in CTC number was observed between groups over time. Platelet cloaking was identified in 29.5% of participants. A positive correlation between CTC number and white cell count (WCC) was observed (p = 0.0001), in addition to a positive relationship between CTC clusters and PSA levels (p = 0.0393).CONCLUSION: The presence of platelet cloaking has been observed in this patient population for the first time, in addition to a significant correlation between CTC number and WCC.TRIAL REGISTRATION: ClincalTrials.gov identifier NCT02453139.

AB - BACKGROUND: Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine the effectiveness of a structured exercise programme in modulating levels of CTCs and platelet cloaking in patients with metastatic prostate cancer.METHODS: Participants (n = 61) were randomised into either standard care (control) or exercise arms. Whole blood was collected for all participants at baseline (T0), three months (T3) and six months (T6), and analysed for the presence of CTCs, CTC clusters and platelet cloaking. CTC data was correlated with clinico-pathological information.RESULTS: Changes in CTC number were observed within group over time, however no significant difference in CTC number was observed between groups over time. Platelet cloaking was identified in 29.5% of participants. A positive correlation between CTC number and white cell count (WCC) was observed (p = 0.0001), in addition to a positive relationship between CTC clusters and PSA levels (p = 0.0393).CONCLUSION: The presence of platelet cloaking has been observed in this patient population for the first time, in addition to a significant correlation between CTC number and WCC.TRIAL REGISTRATION: ClincalTrials.gov identifier NCT02453139.

U2 - 10.1371/journal.pone.0243928

DO - 10.1371/journal.pone.0243928

M3 - Article

C2 - 33338056

SN - 1932-6203

VL - 15

JO - PLoS ONE

JF - PLoS ONE

IS - 12

M1 - e0243928

ER -

Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial

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