Laser desorption of dye-tagged oligonucleotides was studied using laser-induced fluorescence imaging. Desorption with ultra violet (UV) and infra-red (IR) lasers resulted in forward directed plumes of molecules. In the case of UV desorption, the initial shot desorbed approximately seven-fold more material than subsequent shots. In contrast, the initial shot in IR desorption resulted in the ejection of less material compared to subsequent shots and these plumes had a component directed along the path of the laser. Thermal equilibrium of the molecules in the plume was achieved after approximately 25 µs with a spread in molecular temperature which was described by a modified Maxwell-Boltzmann equation.
|Number of pages||10|
|Journal||International Journal of Biological Macromolecules|
|Publication status||Published - 01 May 2012|
ASJC Scopus subject areas
- Molecular Biology
- Structural Biology
Merrigan, T. L., Timson, D. J., Hunniford, C. A., Catney, M., & McCullough, R. W. (2012). Plume characteristics and dynamics of UV and IR laser-desorbed oligonucleotides. International Journal of Biological Macromolecules, 50(4), 1081-90. https://doi.org/10.1016/j.ijbiomac.2012.03.007