Plume characteristics and dynamics of UV and IR laser-desorbed oligonucleotides

Tony L Merrigan, David J Timson, C. Adam Hunniford, Martin Catney, Robert W McCullough

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Laser desorption of dye-tagged oligonucleotides was studied using laser-induced fluorescence imaging. Desorption with ultra violet (UV) and infra-red (IR) lasers resulted in forward directed plumes of molecules. In the case of UV desorption, the initial shot desorbed approximately seven-fold more material than subsequent shots. In contrast, the initial shot in IR desorption resulted in the ejection of less material compared to subsequent shots and these plumes had a component directed along the path of the laser. Thermal equilibrium of the molecules in the plume was achieved after approximately 25 µs with a spread in molecular temperature which was described by a modified Maxwell-Boltzmann equation.
Original languageEnglish
Pages (from-to)1081-90
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume50
Issue number4
DOIs
Publication statusPublished - 01 May 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

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