Polymer-based protein engineering grown ferrocene-containing redox polymers improve current generation in an enzymatic biofuel cell

Alan S Campbell, Hironobu Murata, Sheiliza Carmali, Krzysztof Matyjaszewski, Mohammad F Islam, Alan J Russell

Research output: Contribution to journalArticle

38 Citations (Scopus)
3 Downloads (Pure)

Abstract

Enzymatic biofuel cells (EBFCs) are capable of generating electricity from physiologically present fuels making them promising power sources for the future of implantable devices. The potential application of such systems is limited, however, by inefficient current generation. Polymer-based protein engineering (PBPE) offers a unique method to tailor enzyme function through tunable modification of the enzyme surface with functional polymers. In this study, we report on the modification of glucose oxidase (GOX) with ferrocene-containing redox polymers to increase current generation efficiency in an enzyme-modified anode. Poly(N-(3-dimethyl(ferrocenyl)methylammonium bromide)propyl acrylamide) (pFcAc) was grown from covalently attached, water-soluble initiator molecules on the surface of GOX in a "grafting-from" approach using atom transfer radical polymerization (ATRP). The covalently-coupled ferrocene-containing polymers on the enzyme surface promoted the effective "wiring" of the GOX active site to an external electrode. The resulting GOX-pFcAc conjugates generated over an order of magnitude increase in current generation efficiency and a 4-fold increase in maximum EBFC power density (≈1.7µWcm(-2)) with similar open circuit voltage (0.27V) compared to native GOX when physically adsorbed onto paddle-shaped electrodes made up of electrospun polyacrylonitrile fibers coated with gold nanoparticles and multi-wall carbon nanotubes. The formation of electroactive enzyme-redox polymer conjugates using PBPE represents a powerful new tool for the improvement of mediated enzyme-based bioelectronics without the need for free redox mediators or anode/cathode compartmentalization.

Original languageEnglish
Pages (from-to)446-453
JournalBiosensors and Bioelectronics
Volume86
DOIs
Publication statusPublished - 15 Dec 2016
Externally publishedYes

Bibliographical note

Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

Keywords

  • Acrylic Resins/chemistry
  • Aspergillus niger/enzymology
  • Bioelectric Energy Sources/microbiology
  • Electricity
  • Electrodes
  • Ferrous Compounds/chemistry
  • Glucose Oxidase/chemistry
  • Metallocenes
  • Oxidation-Reduction
  • Polymerization
  • Polymers/chemistry

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