Porcine circovirus 2 infection in swine foetuses inoculated at different stages of gestation

Romeo E. Sanchez; Hans J. Nauwynck; Francis McNeilly; Gordon M. Allan; Maurice B. Pensaert

doi:10.1016/S0378-1135(01)00425-4

Porcine circovirus 2 infection in swine foetuses inoculated at different stages of gestation

Romeo E. Sanchez, Hans J. Nauwynck, Francis McNeilly, Gordon M. Allan, Maurice B. Pensaert^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

90 Citations (Scopus)

Abstract

The ability of porcine circovirus 2 (PCV2) to replicate and cause pathologic abnormalities in foetuses at selected time points of gestation was examined in this study. Two foetuses were inoculated in utero in each of two sows at 57, 75 and 92 days of gestation, respectively, with PCV2 (1121). The remaining foetuses were left uninoculated to assess whether intra-uterine spread occurred. Twenty-one days after inoculation, the foetuses were collected and examined for gross lesions and for virus and infected cells in different organs. Serum samples from all foetuses were tested for PCV2 antibodies. Virus replication was detected in all inoculated foetuses. Spread to non-inoculated foetuses did not occur. Virus replication was significantly higher in foetuses inoculated at 57 days compared to that inoculated at 75 and 92 days. The heart contained the highest virus titre and highest number of viral antigen positive cells. Gross lesions were observed only in foetuses inoculated at 57 days of age. PCV2 antibodies were detected only in foetuses inoculated at 75 and 92 days. This study shows the ability of PCV2 to replicate in foetuses at different stages of gestation and to cause pathologic abnormalities in foetuses inoculated at 57 gestational days.

Original language	English
Pages (from-to)	169-176
Number of pages	8
Journal	Veterinary Microbiology
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1135(01)00425-4
Publication status	Published - 08 Nov 2001
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank J. Ellis for kindly supplying the PCV2 isolate. We also acknowledge S. Van Gucht, G. Labarque and F. de Backer for their assistance during surgery and I. Parreyn, V. Van Hoorde and C. Bracke for their excellent technical support. We thank D. Maes for advice on statistical analysis. Romeo E. Sanchez Jr. is funded by Grant QLK2-CT-1999-00445 from the European Union.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

Foetus
Immunofluorescence
Pig-viruses
Porcine circovirus (PCV)
Virus isolation

ASJC Scopus subject areas

Microbiology
General Veterinary

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10.1016/S0378-1135(01)00425-4

Cite this

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title = "Porcine circovirus 2 infection in swine foetuses inoculated at different stages of gestation",

abstract = "The ability of porcine circovirus 2 (PCV2) to replicate and cause pathologic abnormalities in foetuses at selected time points of gestation was examined in this study. Two foetuses were inoculated in utero in each of two sows at 57, 75 and 92 days of gestation, respectively, with PCV2 (1121). The remaining foetuses were left uninoculated to assess whether intra-uterine spread occurred. Twenty-one days after inoculation, the foetuses were collected and examined for gross lesions and for virus and infected cells in different organs. Serum samples from all foetuses were tested for PCV2 antibodies. Virus replication was detected in all inoculated foetuses. Spread to non-inoculated foetuses did not occur. Virus replication was significantly higher in foetuses inoculated at 57 days compared to that inoculated at 75 and 92 days. The heart contained the highest virus titre and highest number of viral antigen positive cells. Gross lesions were observed only in foetuses inoculated at 57 days of age. PCV2 antibodies were detected only in foetuses inoculated at 75 and 92 days. This study shows the ability of PCV2 to replicate in foetuses at different stages of gestation and to cause pathologic abnormalities in foetuses inoculated at 57 gestational days.",

keywords = "Foetus, Immunofluorescence, Pig-viruses, Porcine circovirus (PCV), Virus isolation",

author = "Sanchez, \{Romeo E.\} and Nauwynck, \{Hans J.\} and Francis McNeilly and Allan, \{Gordon M.\} and Pensaert, \{Maurice B.\}",

note = "Funding Information: The authors thank J. Ellis for kindly supplying the PCV2 isolate. We also acknowledge S. Van Gucht, G. Labarque and F. de Backer for their assistance during surgery and I. Parreyn, V. Van Hoorde and C. Bracke for their excellent technical support. We thank D. Maes for advice on statistical analysis. Romeo E. Sanchez Jr. is funded by Grant QLK2-CT-1999-00445 from the European Union. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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AU - Pensaert, Maurice B.

N1 - Funding Information: The authors thank J. Ellis for kindly supplying the PCV2 isolate. We also acknowledge S. Van Gucht, G. Labarque and F. de Backer for their assistance during surgery and I. Parreyn, V. Van Hoorde and C. Bracke for their excellent technical support. We thank D. Maes for advice on statistical analysis. Romeo E. Sanchez Jr. is funded by Grant QLK2-CT-1999-00445 from the European Union. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

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N2 - The ability of porcine circovirus 2 (PCV2) to replicate and cause pathologic abnormalities in foetuses at selected time points of gestation was examined in this study. Two foetuses were inoculated in utero in each of two sows at 57, 75 and 92 days of gestation, respectively, with PCV2 (1121). The remaining foetuses were left uninoculated to assess whether intra-uterine spread occurred. Twenty-one days after inoculation, the foetuses were collected and examined for gross lesions and for virus and infected cells in different organs. Serum samples from all foetuses were tested for PCV2 antibodies. Virus replication was detected in all inoculated foetuses. Spread to non-inoculated foetuses did not occur. Virus replication was significantly higher in foetuses inoculated at 57 days compared to that inoculated at 75 and 92 days. The heart contained the highest virus titre and highest number of viral antigen positive cells. Gross lesions were observed only in foetuses inoculated at 57 days of age. PCV2 antibodies were detected only in foetuses inoculated at 75 and 92 days. This study shows the ability of PCV2 to replicate in foetuses at different stages of gestation and to cause pathologic abnormalities in foetuses inoculated at 57 gestational days.

AB - The ability of porcine circovirus 2 (PCV2) to replicate and cause pathologic abnormalities in foetuses at selected time points of gestation was examined in this study. Two foetuses were inoculated in utero in each of two sows at 57, 75 and 92 days of gestation, respectively, with PCV2 (1121). The remaining foetuses were left uninoculated to assess whether intra-uterine spread occurred. Twenty-one days after inoculation, the foetuses were collected and examined for gross lesions and for virus and infected cells in different organs. Serum samples from all foetuses were tested for PCV2 antibodies. Virus replication was detected in all inoculated foetuses. Spread to non-inoculated foetuses did not occur. Virus replication was significantly higher in foetuses inoculated at 57 days compared to that inoculated at 75 and 92 days. The heart contained the highest virus titre and highest number of viral antigen positive cells. Gross lesions were observed only in foetuses inoculated at 57 days of age. PCV2 antibodies were detected only in foetuses inoculated at 75 and 92 days. This study shows the ability of PCV2 to replicate in foetuses at different stages of gestation and to cause pathologic abnormalities in foetuses inoculated at 57 gestational days.

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KW - Immunofluorescence

KW - Pig-viruses

KW - Porcine circovirus (PCV)

KW - Virus isolation

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JF - Veterinary Microbiology

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ER -

Porcine circovirus 2 infection in swine foetuses inoculated at different stages of gestation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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